微囊藻毒素的极谱测定法  

Determination of microcystin in water with polarography

作　　者：刘斌[1] 周培疆[1] 宋立荣[2] 

机构地区：[1]武汉大学环境科学系,湖北武汉430079 [2]中国科学院水生生物研究所,湖北武汉430072

出　　处：《中国环境监测》2005年第1期33-37,共5页Environmental Monitoring in China

基　　金：国家高技术研究发展计划 ( 863)项目 (No.2 0 0 1AA64 1 0 30 ) ;国家自然科学基金项目 (No.2 0 1 770 1 8)

摘　　要：建立了微囊藻毒素的一种新的测定方法。在Ni2 + 硼砂为支持电解质的体系中 ,微囊藻毒素 RR在 -1 5 5V产生一个极谱波。在此实验条件下 ,微囊藻毒素在 1 5 0～8 0 0mg L浓度范围内 ,极谱波高与微囊藻毒素浓度有良好的线性关系 ,线性相关系数为 0 998,检测限为 0 0 7mg L。同时对测定条件进行了研究 ,并探讨了极谱电流的性质 ,认为该极谱波是一个催化氢波 ;常见无机物和有机物不干扰本法测定 ,具有良好的选择性。该方法用于实际水样测定 。This paper brings up a new analytic method of Microcystins. Microcystin_RR can produce a sensitive catalytic hydrogen wave in 3×10^(-4)mol/L Ni^(2+)-2×10^(-2)mol/L borax medium, E_(p) is -1.55V(vs. SCE). The good linear concentration of Microcystin-RR with derivative peak current is over the range of 1.50～8.00mg/L. The detection limit of the method is 0.07mg/L. The conditions of the analysis were studied and the mechanism of electrode reaction was explored. The result shows that its catalytic wave to hydrogen is coordination compound of microcystin and Ni^(2+), which catalysis hydrogen to discharge. This method has been applied to the analysis of water sample and the result is satisfying.

关 键 词：微囊藻毒素 单扫描示波极谱法 催化氢波 

分 类 号：X835[环境科学与工程—环境工程]