反相高效液相色谱法测定肠安胶囊中黄芩苷的含量  

Content Determination of Baicalin in Chang'an Capsule by RP-HPLC

作　　者：邵家德[1] 周琴妹[1] 陈晓斌[1] 朱政中[1] 徐燕[1] 沈洪[1] 

机构地区：[1]江苏省中医院,南京市210029

出　　处：《中国药房》2005年第5期378-379,共2页China Pharmacy

摘　　要：目的 :建立以反相高效液相色谱法测定肠安胶囊中黄芩苷含量的方法。方法 :色谱柱为C18,流动相为甲醇 -0 07 %磷酸溶液 (44∶56),流速为1ml/min ,检测波长为280nm ,柱温为室温。结果 :黄芩苷进样量在0 1054μg～1 0540μg范围内与峰面积呈良好的线性关系 (r=0 9996) ,平均加样回收率为97 44 % (RSD=2 92 % ,n=5)。结论 :本方法简便、准确、快速 ,可用于该制剂的含量测定。OBJECTIVE:To establish the method for determination of baicalin in Chang’an capsule by RP-HPLC. METHODS:HPLC was performed on C 18 column with methanol-0.07%phosphoric acid solution(44∶56)as mobile phase at a flow rate of lml/min and the temperature of column kept at room temperature.The UV detection wavelength was280nm.RESULTS:The linear range of baicalin was sample size0.1054μg～1.0540μg(r=0.9996),the average recovery was97.44%(RSD=2.92%,n=5).CONCLUSION:The method was simple,accurate and fast,and can be used for the determination of baicalin.

关 键 词：反相高效液相色谱法 肠安胶囊 黄芩苷 含量测定 

分 类 号：R927.2[医药卫生—药学] R944.5