酶底物对硝基苯-α-D-吡喃葡糖苷的合成  

Synthesis of an enzyme-substrate p-nitrophenyl-α-D-glucopyranoside

作　　者：孙家莉[1] 褚婕[1] 丁晓琴[1] 

机构地区：[1]天津市医药科学研究所,天津300020

出　　处：《化学试剂》2005年第2期97-98,共2页Chemical Reagents

基　　金：天津市卫生局科技基金资助项目 (99KY0 42 )。

摘　　要：以葡萄糖为原料 ,经乙酰化、缩合、分离、脱乙酰化 4步反应 ,合成了临床检验需要的α 葡糖苷酶底物———对硝基苯-α -D-吡喃葡糖苷。所合成的底物通过红外、紫外、核磁、旋光和元素分析确认了结构。p-Nitrophenyl-α-D-glucopyranoside(PNP- α-G)was prepared for the determination of α-gluconidase activity in clinical examination.PNP-α-G was obtained by using D-glucose as starting material through four steps:acetylation,condensation,separation and deacetylation.The fusion method and solvent method were compared in the condensation.Structure of the substrate was identified by IR,UV, 1HNMR,polarimetry and elemental analysis.

关 键 词：对硝基苯-α-D-吡喃葡糖苷 合成 Α-葡糖苷酶 底物 

分 类 号：O629.13[理学—有机化学]