广东省女性吸毒者中流行的HIV-1基因序列分析  

Subtype and sequence analysis of the env gene in HIV-1 infected female drug users in Guangdong.

作　　者：颜瑾[1] 王玉[2] 李杰[1] 马鹏飞[2] 林鹏[1] 邢辉[2] 刁丽梅[1] 邵一鸣 

机构地区：[1]广东省疾病预防控制中心,广州510300 [2]中国疾病预防控制中心性病艾滋病预防控制中心,北京100050

出　　处：《中国艾滋病性病》2005年第1期9-12,共4页Chinese Journal of Aids & STD

基　　金：广东省医学科学技术研究基金(WSTJJ20011226440105197202120061)

摘　　要：目的了解广东省女性吸毒者中艾滋病病毒1型(HIV1)亚型的流行规律及其与国际参考株的同源性。方法应用套式聚合酶链反应对20例广东省感染了HIV1的女性吸毒者淋巴细胞富集液的核酸样品进行扩增,并使用ABI377型测序仪对扩增产物测序后,对其env基因C～V段的核酸序列进行比较分析。结果20份血样中,9份为07BC重组亚型,与国际标准株CN97C54A最近,基因离散率为233%±1318,组内离散率为3307%±1483;6份为AE重组亚型,与国际标准株01AETH90CM240最近,基因离散率为8607%±0917,组内离散率为6934%±4560;4份为08BC重组亚型,与国际标准株97CNGX9F最近,基因离散为2708%±1593,组内离散率为3655%±1197;1份为C亚型,与国际标准株95IN21068最近,基因距离为568%。结论广东省女性吸毒者中既存在主要在广东省、广西壮族自治区吸毒人群中流行的08BC重组亚型,又存在主要在西北地区吸毒人群中流行的07BC重组亚型,也存在主要在性途径感染人群中流行的AE重组亚型,提示在女性吸毒者中流行的亚型趋于复杂,女性吸毒者作为HIV从吸毒人群流向一般人群的桥梁作用正在显现,应尽快采取有效措施,防止HIV的进一步蔓延。Objective To determine the subtype of HIV-1 in female drug users in Guangdong Province and its homogeneity with international reference strains.Methods HIV-1 pro-viral DNA from 20 female drug users in Guangdong was amplified by nested PCR.The C2-V3 regions of HIV-1 env gene was sequenced directly from the PCR product and analyzed.Results Twenty samples from the female drug users were confirmed to be infected with four HIV-1 subtypes.Of them 9 samples were of 07-BC,being closest to the international standard strain CN97.C54A,isolated in northeastern China,with the gene discrete time rate of 2.33%±1.318 and 3.307%±1.483 within the genome;6 samples were AE,being closest to the international standard strain 01AE.TH.90.CM240 isolated in Thailand,with the gene discrete time rate of 8.607%±0.917 and 6.934%±4.650;4 samples were 08-BC,being closest to the international standard strain 97CNGX-9F isolated in Guangxi Province of China,with the discrete time rate of 2.708%±1.593 and 3.655%±1.197;and 1 sample was C subtype,being closest to the international standard strain 95IN21068 isolated in India,with a gene distance of 5.68%.Conclusion HIV-1 subtypes were complicated in female drug users in Guangdong.Measures should be taken to prevent HIV spreading from drug users to the general population through them.

关 键 词：女性吸毒者 艾滋病病毒1型亚型 序列测定 

分 类 号：R512.91[医药卫生—内科学]