铍试剂Ⅱ线性扫描伏安探针法测定蛋白质  

Linear Sweep Voltammetric Determination of Protein with Beryllon Ⅱ as Probe

作　　者：孙伟[1] 韩军英[1] 焦奎[1] 陆路德[2] 

机构地区：[1]青岛科技大学化学与分子工程学院,青岛266042 [2]南京理工大学化工学院

出　　处：《应用化学》2005年第3期242-245,共4页Chinese Journal of Applied Chemistry

基　　金：中国博士后科学基金(2003033492);国家自然科学基金(20405008; 20375020)资助项目

摘　　要：根据在pH=4.3的Britton-Robinson(B-R)缓冲溶液中铍试剂Ⅱ与蛋白质能够反应生成稳定的超分子复合物,使铍试剂Ⅱ在-0.48 V(vs.SCE)处的伏安还原峰峰电流下降而峰电位基本保持不变的性质,建立了蛋白质的二阶导数线性扫描伏安测定方法. 在最佳条件下,峰电流的下降值同人血清白蛋白(HSA)的浓度在5.0～50.0 mg/L范围内呈线性关系,线性回归方程为Δi″p(nA)=49.83+3.65c(mg/L),γ=0.996 6. 此法应用于实际人血清样品的测定,结果与经典的考马斯亮蓝G-250光度法一致.Beryllon Ⅱ interacts with protein to form a supermolecular complex in a pH 4.3 Britton-Robinson(B-R) buffer solution and produces a sensitive reduction peak at -0.48 V(vs.SCE) on the hanging mercury drop electrode. According to the decrease of the reduction peak current without the shifting of the peak p otential of beryllon Ⅱ after the addition of human serum albumin(HSA) to the above solution, a new linear sweep voltammetric determination method for protein is proposed. Under the optimal electrochemical conditions, the reduction peak c urrent of beryllon Ⅱ decreases linearly with the HSA concentration ranged from 5.0 to 50.0 mg/L and the linear regression equation is Δi″_p(nA )=49.83+3.65c(mg/L), γ=0.996 6. The method was applied to determ ine the content of HSA in human blood samples and the result was in good agree ment with that obtained by the traditional Coomassie brilliant blue G-250 spe ctrophotometric method. The method was also used to determine bovine serum album in, bovine hemoglobin with satisfactory results.

关 键 词：蛋白质 铍试剂Ⅱ 线性扫描伏安法 

分 类 号：O657.1[理学—分析化学]