抗细粒棘球蚴B抗原人源单链可变区抗体临床诊断价值的初步研究  

Initial Clinical Study on Single Chain Fragment of Variable Antibody Against Antigen B of Echinococcus granulosus

作　　者：陈新华[1] 温浩[2] 张金辉[2] 林仁勇[2] 张琰[2] 张静萍[2] 郑树森[1] 

机构地区：[1]浙江大学医学院第一附属医院,杭州310031 [2]新疆医科大学第一附属医院,乌鲁木齐830054

出　　处：《地方病通报》2005年第1期1-3,共3页Endemic Diseases Bulletin

基　　金：国家自然科学基金(30160081);新疆维吾尔自治区自然科学基金项目(200221101)

摘　　要：目的　评价人源单链可变区抗体(ScFv)诊断细粒棘球蚴病的临床价值。方法　用Western-blot检测ScFv识别的抗原表位,并检测该抗体与包虫病病人囊液、血清中循环抗原的结合,与其它肝占位疾病患者血清、正常人血清的交叉反应。结果　ScFv识别囊液抗原位点单一,特异性优于多克隆抗体,交叉反应低,但检测囊液及血清循环抗原的敏感性低于多克隆抗体。结论　对临床使用ScFv诊断进行了初步尝试,直接ELISA法检出率不高,应改进为双抗体夹心法或多种抗体铺底捕获法来检测循环抗原。Objective To evaluate the clinical diagnostic effect of Single Chain Fragment of Variable Antibody(ScFv) against Antigen B of Echinococcus granulosus. Methods Western-blot was applied to compare epitopes recognized by ScFv and polyclonal antibody. 17 cyst fluid samples, 56 positive blood samples, 33 other nonhydatid blood samples and 37 normal control blood samples were examined by polyclonal antibody and ScFv with direct ELISA. Results ScFv can recognize 27kD protein in cyst fluid and its diagnostic speciality is higher than that of polyclonal antibody, and almost has no cross reaction. While its sensitivity is much lower than that of polyclonal antibody. Conclusions ScFv shows good speciality but poor sensitivity in the serological diagnosis, so other methods of ELISA should be tried to promote the clinical uses of ScFv.

关 键 词：细粒棘球蚴B抗原 单链可变区抗体 诊断 

分 类 号：R383.33[医药卫生—医学寄生虫学] R446.6[医药卫生—基础医学]