清热中药对高糖培养下肾小球系膜细胞增殖的影响  

Effects of Heat-Clearing Herbs on the Proliferation of Glomeruli Mesangial Cells Induced by High Glucose

作　　者：孙敏[1] 孙晶[2] 朱荃[3] 

机构地区：[1]安徽大学生命科学院,安徽合肥230029 [2]江苏大学,2004级研究生江苏镇江212013 [3]南京中医药大学国家规范化中药药理实验室,江苏南京210029

出　　处：《上海中医药杂志》2005年第3期58-59,共2页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家科技部十五攻关课题(2001BA701A29)

摘　　要：为研究清热中药治疗糖尿病肾病的作用机理,验证中医理论对糖尿病发病机制的认识,并为临床用药提供依据。采用MTT比色法,观察具有清热作用的中药知母、生地、胡黄连和地骨皮水提物对体外高糖条件下培养的牛肾小球系膜细胞增殖的影响。结果显示,知母、生地和胡黄连对系膜细胞增殖有抑制作用,而地骨皮无明显作用。提示清热中药可能通过抑制系膜细胞的增殖从而对糖尿病肾病的发生产生一定的影响。To study the mechanism of heat-clearing herbs in treating diabetic nephropathy and verify the theory of traditional Chinese medicine in the episode of diabetic nephropathy for clinical evidence, the glomeruli mesangial cells proliferation were induced by high glucose incubation in vitro and the effect of herbs on the proliferation were detected by MTT assay. Results: Radix Rehmanniae, Rhizoma Anemarrhenae and Picrorhiza Rhizome could inhibit the proliferation induced by high glucose, but Cortex lycil could not do. It is revealed that heat-clearing herbs can inhibit the genesis of diabetic nephropathy.

关 键 词：糖尿病肾病 系膜细胞 增殖作用 

分 类 号：R285.5[医药卫生—中药学]