HPLC法测定当归四逆汤中芍药苷的含量  

Determination of Paeoniflorin in Dangguisini Granules by HPLC

作　　者：丁野[1] 黄罗生[2] 高展[2] 

机构地区：[1]湖南省药品检验所,长沙410001 [2]中国药科大学中药研究所中药制剂研究室,南京210038

出　　处：《海峡药学》2005年第1期37-39,共3页Strait Pharmaceutical Journal

摘　　要：目的 　建立 HPLC测定当归四逆汤中芍药苷的定量方法。 方法 　以反相高效液相法测定芍药苷的含量。固定相 :Shimpack CL C-ODS(15 0 mm× 6mm) ,流动相 :乙腈 -0 .1%磷酸水溶液 (17∶ 83 ) ,检测波长 :2 3 0 nm。 结果 　芍药苷在 9.1-91μg· m L- 1范围内线性良好 (r=0 .9999) ,平均加样加收率为 10 0 .3 6% ,RSD为 1.91%。 结论 　该方法简便准确 ,专属性强 ,能满足该制剂质量标准的要求。OBJECTIVE The purpose is to develop the method for determining the contert of Paeoniflorin.METHODS The Paeoniflorin in Dangguisini granules was determined by HPLC. The separation was performed on Shimpack CLC-ODS column with Acetonitrile-0.1% phosphate acid(17∶83)as mobile phase.Paeoniflorin was detected at 230nm.RESULTS The calibration curve was linear (r=0.9999)in the range of 9.1-91μg·mL -1 for Paeoniflorin. The average recovery was 100.36% and RSD was 1.91%.CONCLUSION The method was simple, specific and accurate, and can effectively control quality of Dangguisini granules.

关 键 词：当归四逆汤 芍药苷 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]