原子力显微镜对生理溶液中活细胞成像条件的研究  

Study on the basic conditions affecting the atomic force microscopic observation of the living cells in physical solutions

作　　者：王莉娟[1] 张英鸽[1] 张飒[1] 张德添[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《电子显微学报》2005年第1期79-84,共6页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金资助项目(No 30271501). ~~

摘　　要：本文研究用原子力显微技术(AFM)在生理条件下对活细胞成像的基本方法,并对各种影响成像因素如针尖与细胞表面的非特异性相互作用、AFM悬臂弹性系数及细胞表面柔性等问题提供相应的解决方案。从而为AFM在成像的基础上对活细胞其它性质的研究提供基础。用本文方法清晰地显示了固定细胞与活细胞膜表面所具有的明显差别:活细胞膜完整平滑,固定细胞表面粗糙,边缘不整。The basic methods to image living cells in physiological solutions with atomic force microscope (AFM) and various factors that affect the quality of the image have been studied. These factors include the non-specific interactions between the AFM tip and the surface of the cells, the elastic constant of the cantilever, the softness of the cells and so on. Several ways have been proposed to solve the problems often encountered in AFM imaging of the living cells in physiological solutions, which provided basis for the studies on the other surfacial properties of the living cells. With the method reported in this paper, the membrane surface of the living cells can be clearly distincted from that of the fixed cells. The membrane of the living cells is intact and smooth while that of the fixed cells is rough and incomplete at their margin.

关 键 词：细胞表面 活细胞 成像条件 固定 非特异性 生理 平滑 原子力显微技术 AFM 原子力显微镜 

分 类 号：Q2-3[生物学—细胞生物学]