当归、川芎中藁本内酯含量测定及醇提工艺研究  

Study on the determination and extraction of ligustilide in Radix Angelicae sinensis and Rhizoma chuanxiong

作　　者：胡杰[1] 冯丽莉[1] 刘延[1] 崔福德[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2005年第2期145-148,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的研究当归、川芎中藁本内酯醇提的最佳工艺条件。方法建立HPLC法测定提取液中指标成分藁本内酯的含量 ,并用正交设计优化醇提工艺条件。结果与结论用甲醇 - 水 - 乙酸(V∶V∶V =75 . 0∶2 4 . 8∶0 . 2 )作流动相能使藁本内酯得到良好的分离 ;8倍量 85 % (φ)乙醇提取3次 ,每次 1 0h为醇提最佳工艺条件。Objective To optimize the extraction process of ligustilide in Radix Angelicae sinensis and Rhizoma chuanxiong.Methods The HPLC assay method was established to determine the content of ligustilide(LD) in the solution extracted,and the extraction process of LD with alcohol was studied and optimized by the orthogonal design.Results and Conclusion Ligustilide was well isolated with a mobile phase of methanol-H 2O-HOAc(V∶V∶V=75.0∶24.8∶0.2); the optimum extraction process was adding 8 times amount of 85%(φ)alcohol,refluxing and extracting for 3 times, 1.0 h each time.

关 键 词：当归 川芎 藁本内酯 高效液相色谱 正交设计 

分 类 号：R944.9[医药卫生—药剂学]