溴酸钾氧化吖啶黄催化褪色光度法测定痕量亚硝酸根的研究  

PHOTOMETRIC DETERMINATION OF TRACE AMOUNTS OF NITRITE BY CATALYSIS OF OXIDATION OF ACRIDINE YELLOW BY POTASSIUM BROMATE

作　　者：司文会[1] 李克俭[1] 李永兵[2] 

机构地区：[1]苏州农业职业技术学院,苏州215008 [2]安徽技术师范学院,凤阳233100

出　　处：《理化检验（化学分册）》2005年第3期194-196,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：苏州农业职业技术学院自然科学研究基金资助项目(ZR20040014)

摘　　要：研究了在 0.10 mol·L-1的盐酸介质中,亚硝酸根催化溴酸钾氧化伯胺基结构碱性染料吖啶黄褪色反应的适宜条件与影响因素,建立了催化动力学褪色光度法测定痕量亚硝酸盐的新方法。亚硝酸根浓度在0.25~20.00μg/25 mL范围内符合比耳定律,其λmax=455nm,Sandell灵敏度为36.20 ng·cm-2,RSD为1.15%~5.97%,回收率为 97.5%~105.0%。方法用于环境样品、生物材料、食品等实际样品中亚硝酸根的测定,试剂简单,方法快速,选择性好,结果满意。The oxidation of acridine yellow (a basic dyestuff bearing a primary amine structure in its molecule) by potassium bromate in a slightly acidic solution of 0.10 mol·L^(-1) HCl, was catalyzed by traces of NO^-_2-ions, leading to decolorization of acridine yellow. The magnitude of the intensity of color-fading was found to keep a linear relationship with the concentration of NO^-_2 in the range of 0.25~20.00 μg per 25 mL of solution, with the absorbance measured at 455 nm. Sandell′s sensitivity of this color-fading oxidation reaction was 36.20 ng·cm^(-2). Precision and accuracy of this method were tested, and values of RSD and recoveries were in the range of 1.15%~5.97% and 97.5%~105.0%, respectively. This method has been used in the analysis of environmental samples, biological materials and foodstaffs, giving satisfactory results.

关 键 词：催化褪色光度法 吖啶黄 亚硝酸根 溴酸钾 环境试样 生物材料 食品 

分 类 号：O657.38[理学—分析化学]