协同增敏荧光光度法在双嘧达莫分析中的应用  

APPLICATION OF SYNERGIC FLUOROPHOTOMETRY TO THE ANALYSIS OF DIPYRIDAMOLE

作　　者：童金强[1] 上官云凤[1] 喻克雄[1] 

机构地区：[1]长江大学化学与环境工程学院,荆州434020

出　　处：《理化检验（化学分册）》2005年第3期197-198,202,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：湖北省教育厅科学研究项目(2003A003)

摘　　要：用荧光光度分析法研究了三羟甲基氨基甲烷和十二烷基硫酸钠对双嘧达莫的协同荧光增敏作用,提出了在此条件下测定双嘧达莫的协同荧光增敏光度法。方法灵敏度高,检出限低(9.20×10-9mol·L-1),在 7.927×10-8~2.378×10-6 mol·L-1范围内荧光强度与双嘧达莫的浓度呈良好线性关系。It was found that the self-emitting fluorescence of dipyridamole (DIP) can be enhanced synergically by sodium dodecyl sulfonate (SDS) and trihydroxymethylaminomethane [(CH_2OH)_3CNH_2, THMAM]. A thorough study on this phenomenon was made both of its optimum reaction conditions and its mechanism of synergic sensitization action. Linear relationship between the magnitude of fluorescence intensity (λ_(ex) 291 nm, λ_(em) 483 nm) and the concentration of DIP was obtained in the range of 7.927×10^(-8)~2.378×10^(-6)mol·L^(-1) (DIP) having the regression equation F=20.11×10^(-7)C_(DIP)-14.43 (r=0.999 7). The detection limit was found to be 9.20×10^(-9)mol·L^(-1). In the application of this fluorophotometric method to the determination of DIP in serum samples, recoveries in the range of 98.9% to 100.8% were obtained.

关 键 词：荧光光度法 协同荧光增敏 双嘧达莫 药物分析 

分 类 号：O657.39[理学—分析化学]