应用间接ELISA检测鸡痘病毒抗体方法的建立  

Development of Indirect ELISA for Detection of Antibodies to Fowlpox Virus

作　　者：张体银[1] 李军伟[1] 孙蕾[1] 孙学辉[1] 张如宽[1] 刘秀梵[1] 

机构地区：[1]扬州大学农业部畜禽传染病学重点开放实验室,江苏扬州225009

出　　处：《中国兽医学报》2005年第2期128-130,共3页Chinese Journal of Veterinary Science

基　　金：国家"863"计划项目(2001AA213041);科技部农业科技成果转化资金项目(OIEFN213201078)

摘　　要：建立了检测鸡痘病毒抗体的间接 EL ISA方法。应用该方法检测鸡痘阳性血清 ,其灵敏度为琼脂扩散试验的 4 0 0~ 80 0倍 ,而且还具有特异性强、操作简便。An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to fowlpox virus was developed and compared with agar gel diffusion technique. These assays were used to examine the sera from chickens vaccinated experimentally, and the results revealed that the ELISA was 400-800 times more sensitive than AGID. It was simple, convenient, specific, sensitive and rapid for detecting of antibody to fowlpox virus.

关 键 词：间接ELISA 鸡痘病毒 抗体检测 鸡痘 

分 类 号：S858.31[农业科学—临床兽医学] S852.659.1[农业科学—兽医学]