归芪益母汤耐缺氧及抗氧化作用  

Effect of Gui Qi Yimu Decoction on Hypoxia Tolerance and Anti-oxidation in Mice

作　　者：许小琴[1] 韦旭斌[1] 刘学忠[2] 李金贵[2] 胡仲明[1] 柳巨雄[1] 王治[1] 张玉国[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]扬州大学畜牧兽医学院,江苏扬州225009

出　　处：《中国兽医学报》2005年第2期208-210,共3页Chinese Journal of Veterinary Science

基　　金：解放军总后勤部军需部资助项目(JX990119)

摘　　要：归芪益母汤经水提醇沉制成注射液 ,给小鼠经腹腔注射给药 ,进行常压缺氧、减压缺氧、化学法缺氧和抗氧化试验 ,探讨了归芪益母汤对小鼠耐缺氧和抗氧化能力的影响。结果表明 ,归芪益母汤注射液显著延长了缺氧小鼠的存活时间 (P<0 .0 1 ) ,其中常压缺氧存活时间延长 1 1 0 .2 1 %,减压缺氧存活时间延长 76 .4 8%,化学法缺氧存活时间延长2 4 0 .87%,提示归芪益母汤能够提高小鼠的耐缺氧能力。抗氧化方面 ,体内试验表明 ,其能显著降低小鼠因急性缺氧再暴露所引起的 MDA的产生 (P<0 .0 1 ) ;体外试验表明 ,能拮抗 H2 O2 诱导的溶血 ,抑制超氧离子 (O- 2 )To explore the effects of Gui Qi Yimu decoction on hypoxia tolerance and anti-oxidation,the aqueous extracts of Gui Qi Yimu decoction were once-alcohol deposited and made into injection for this study.The experimental mice were injected intraperitoneally to conduct normobaric hypoxia,hypobaric hypoxia,chemical hypoxia and anti-oxidation experiments.The results showed that Gui Qi Yimu injection significantly prolonged the survival time of experimental mice(P<0.01).The survival time in the mice treated with normobaric hypoxia prolonged by 110.21%,76.48% in the mice treated with hypobaric hypoxia and 240.87% in the mice treated with chemical hypoxia.This suggested Gui Qi Yimu decoction may enhance the ability of hypoxia tolerance.In the aspect of anti-oxidation,the results in vivo showed,the Gui Qi Yimu injection could significantly decrease the synthesis of serum MDA induced by acute hypoxia and re-expose treating(P<0.01).The data in vitro indicated that the hemolysis induced by H_2O_2 and production of O^-_2 could be antagonized or inhibited by Gui Qi Yimu decoction.

关 键 词：归芪益母汤 中药复方 耐缺氧作用 抗氧化作用 小鼠 

分 类 号：S853.76[农业科学—临床兽医学]