4,5-二溴邻硝基苯基荧光酮分光光度法测定微量锌  

Spectrophotometric Determination of the Trace Amount of Zinc(Ⅱ) with 4,5-Dibromo-2-nitro-phenylfluorone(DB-o-NPF)

作　　者：代钢[1] 敖登高娃[1] 乌兰图亚[2] 

机构地区：[1]内蒙古大学化学化工学院,呼和浩特010021 [2]内蒙古农业大学基础部,呼和浩特010019

出　　处：《内蒙古大学学报（自然科学版）》2005年第2期235-237,共3页Journal of Inner Mongolia University：Natural Science Edition

摘　　要：研究了4,5-二溴邻硝基苯基荧光酮(DB-o-NPF)与锌的反应条件及应用.结果表明,在pH为10.5的硼砂-NaOH缓冲溶液中,阳离子表面活性剂溴代十六烷基吡啶(CPB)存在下,Zn( )与DB-o-NPF生成1∶4蓝色配合物,配合物至少稳定4h,最大吸收在624nm波长处,表观摩尔吸光系数为ε=1.13×105L·mol-1·cm-1,锌浓度在0～0.64μg/mL范围服从比尔定律,方法灵敏,操作简便,用于葡萄糖酸锌片、加锌盐等实物中锌的分析测定,回收率测定值在95%～105%之间.并以原子吸收法相对照,结果较一致.The reacting condition and application of DB-o-NPF and Zn(Ⅱ) were studied. The results show that with the presence of CPB,the color reagent reacts with Zn(Ⅱ) and forms a stable blue complex in the medium of Sodium Traborate -NaOH buffer solution (pH=10.5).It remains stable for 4 hours at least.The apparent light absorption coefficient per mol ε=1.13×10~5L·mol^(-1)·cm^(-1) and the maximum absorption happens at λ=624 nm.Beer′s law is obeyed in the concentration range of 0～0.64 μg/mL for Zn(Ⅱ).The proposed method has good sensitivity and selectivity.It has been applied to the determination of trace amount of Zn(Ⅱ) in zinc gluconate tablet and table salt of added zinc with satisfactory results.A better consistency was obtained through a comparison between the determination results of this proposed method and the AAS method.

关 键 词：锌 4 5-二溴邻硝基苯基荧光酮 分光光度法 葡萄糖酸锌片 

分 类 号：O657.32[理学—分析化学]