毛细管区带电泳中钴、镍、锌、锰等金属离子的络合富集  

Chelating Concentration of Cobalt, Nickel, Zinc and Manganese Cations in Capillary Zone Eletrophoresis

作　　者：张凌怡[1] 张维冰[1] 段继诚[1] 张玉奎[1] 

机构地区：[1]中国科学院大连化学物理研究所国家色谱研究分析中心,大连116011

出　　处：《分析化学》2005年第3期305-308,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目(No.20105006)

摘　　要：提出了CZE络合富集的基本方法。通过络合作用使金属离子在样品区带和背景电解质中具有不同迁移速度而实现富集。以金属离子Co2+、Zn2+、Mn2+和Ni2+为溶质,4(2吡啶偶氮)间苯二酚(PAR)和EDTA为络合剂进行实验研究,探讨了柱容量、样品浓度、络合剂种类及进样方式等参数对富集的影响。在不超出柱容量的情况下,峰高随着进样时间的增加而增大。采用pH8.40、6.25mmol/L硼酸盐分离不同浓度Co2+、Ni2+,进样量可增加约100倍,检测灵敏度增加40倍。A method for on-line concentration of metal cation Co2+ , Zn2+, Mn2+ in capillary zone electrophoresis with chelating agent (4-(2-pyridylazo)resorcinol) is presented. In the range of capacity of column, the peak height is heightened along the increasing of injection time. Parameters including capacity of column, sample concentration, kinds of chelator, injection modes were examined. In the separation of Co2+, Ni2+, the injection volume and the detection sensitivity can be increased 100 times and 40 times, respectively with borate buffer (pH 8.40, 6.25 mmoUL) as mobile phase.

关 键 词：进样量 金属离子 络合作用 NI^2+ MN^2+ 富集 CZE 毛细管区带电泳 增加 样品浓度 

分 类 号：O658.9[理学—分析化学]