液-质联用研究红豆杉提取物中紫杉烷类化合物  

Profiling Taxanes in Taxus Extraction Using Liquid Chromatography/Mass Spectrometric Technique

作　　者：李明[1] 陈建民[1] 陈家宽[2] 

机构地区：[1]复旦大学环境科学与工程系,复旦大学天然药物研究中心,上海200433 [2]复旦大学生命科学学院生物多样性科学研究所,生物多样性与生态工程教育部重点实验室,上海200433

出　　处：《分析化学》2005年第3期333-337,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20377008);国家教育部归国留学人员基金资助

摘　　要：建立了一套利用反相高效液相色谱和大气压化学电离质谱联用技术分析红豆杉提取物中微量紫杉烷类化合物的方法。通过研究已知的10种紫杉烷类化合物的保留时间、质谱特征,初步建立了一个紫杉烷类化合物的数据库,利用这些数据可快速鉴定红豆杉提取物中已知的紫杉烷类化合物,并能够对未知化合物做一定程度的结构分析。这种研究方法同样对其他天然产物特别是微量成分结构分析具有指导作用。Analytical method was developed for the trace analysis of taxanes in crude extraction and identification of the antitumor agent paclitaxel. Related taxane analogues have been carried out using a high performance liquid chromatographic. Both diode array detector and mass spectrometry were incorporated into this system, providing additional spectral and structural information for the identification of unknowns samples. Standard taxanes gave excellent positive-ion atmosphere pressure chemical ionization ( APCI) spectra with dominant [ M + H](+) ion in full-scan. Fragmental and structurally informative spectra were obtained at the gave same time. The fragments found were both of the C-13 side-chain and of the taxane ring, so the analysis important information about the taxane structure, including any chemical modification at different positions of the molecular. The data obtained for 10 taxanes using this method provide a taxane profile database useful for the rapid identification of taxanes.

关 键 词：紫杉烷类化合物 提取物 质谱联用技术 红豆杉 反相高效液相色谱 快速鉴定 保留时间 天然产物 

分 类 号：O629[理学—有机化学] O657.63[理学—化学]