反相高效液相色谱检测丹参药材中4种丹参酮的含量  

Simultaneous Determination of Four Kinds of Tanshinone in Salviae Miltiorrhizae Radix by Reversed Phase High-performance Liquid Chromatography

作　　者：张鉴[1] 王兰[2] 袁成凌[1] 王相勤[1] 余增亮[1] 

机构地区：[1]中国科学院离子束生物工程学重点实验室,合肥230031 [2]安徽中医学院,合肥230022

出　　处：《分析化学》2005年第3期355-358,共4页Chinese Journal of Analytical Chemistry

基　　金：安徽省"十五"中药产业化重大专项(01803027)资助课题

摘　　要：建立了测定丹参药材中4种丹参酮含量的反相高效液相色谱法,色谱条件:流动相为水(含0.5%三乙胺)-甲醇-四氢呋喃(45/55/5, V/V/V),流速为1 mL/min;PDA检测波长254 nm;4种成分丹参酮Ⅰ、丹参酮ⅡA、隐丹参酮和二氢丹参酮的加样回收率在95.1%～101.2%之间,线性范围为0.08～2 μg.该方法准确, 稳定,重现性好.根据该色谱条件, 测定了不同产地的丹参药材, 结果表明: 该色谱方法准确检测了生药中4种丹参酮的含量,适合于丹参药材的质量控制.A simple and rapid reversed-phase high-performance liquid chromatography method has been achieved for the determination of tanshinone I, tanshinone II A, dihydrotanshinone and cryptotanshinone in raw Salviae miltiorrhizae radix. This method includes ultrasonic extraction of tanshinones compounds from Salviae miltiorrhizae radix with dichloromethane: ethanol ( 1: 1, V/V). Separation was obtained using a C, column ( 150 mm x 4. 6 mm, 5 mu m) within 20 min. The mobile phase was isocratic conditions with water (containing 0. 5% triethylamine)/methanol/tetrahydrofuran (45/55/5; V/V/V), the flow rate was I mL/min, detection wave length was 254 nm. Four tanshinones compounds were successfully separated from each other, 4 kinds of tanshinones recoveries are in the range of 95. 1% similar to 101. 2%, linear range was from 0. 08 to 2 mu g. In conclusion, the present method has advantages such as simplicity, selectivity and sensitivity as a routine procedure and can be applied to the quality control of the different kinds Salviae miltiorrhizae radix.

关 键 词：丹参酮Ⅰ 含量 色谱条件 药材 生药 不同产地 隐丹参酮 四氢呋喃 甲醇 三乙胺 

分 类 号：TQ461[化学工程—制药化工]