分子印迹化学发光法测定扑热息痛  

Molecular imprinting-chemiluminescence method for determination of paracetamol

作　　者：杜建修[1] 申丽华[1] 吕九如[1] 

机构地区：[1]陕西师范大学化学与材料科学学院,陕西西安710062

出　　处：《陕西师范大学学报（自然科学版）》2005年第1期72-74,共3页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(20275023);陕西省自然科学基金资助项目(2002B12).

摘　　要：采用分子印迹技术合成了对扑热息痛具有选择性识别的高分子聚合物.将此聚合物颗粒填充在化学发光流通池中,当扑热息痛溶液流过流通池时,扑热息痛分子被填充的印迹聚合物选择性俘获;此时,当铁氰化钾和鲁米诺的混合液流过流通池时,吸附的扑热息痛分子与铁氰化钾发生反应而导致铁氰化钾 鲁米诺反应化学发光信号的降低.结果表明,化学发光强度的降低值与扑热息痛浓度的对数值在7～100μmol/L范围内具有线性关系;该方法的检出限为3μmol/L扑热息痛;相对标准偏差小于5%(50.0μmol/L扑热息痛溶液,n=9).该方法已用于尿样中扑热息痛的分析测定.A molecular imprinting-chemiluminescence method for the determination of paracetamol is reported. The method is based on the inhibitory effect of paracetamol, which is selectively adsorbed by paracetamol-imprinted polymer filled in the CL flow cell, on the chemiluminescence reaction of potassium ferricyanide with luminol in alkaline solution. The decreased CL intensity responds linearly with the logarithm of the concentration of paracetamol over 7～100 μmol/L range. The detection limit for paracetamol is 3 μmol/L and the relative standard deviation is less than 5% (50.0 μmol/L paracetamol solution, n=9). The method is applied to the analysis of paracetamol in the diluted urine samples.

关 键 词：扑热息痛 化学发光法 尿样 混合液 鲁米诺 降低 分子印迹 铁氰化钾 选择性识别 俘获 

分 类 号：N37[自然科学总论] O657[理学—分析化学]