鳙鱼线粒体DNA的限制性内切酶图谱  

RESTRICTION ENDONUCLEASE MAP OF mtDNA OF ARISTICHTHYS NOBILIS

作　　者：樊连春[1] 崔建勋[1] 余其兴[1] 

机构地区：[1]武汉大学生命科学学院

出　　处：《武汉大学学报（自然科学版）》1994年第1期121-125,共5页Journal of Wuhan University(Natural Science Edition)

摘　　要：用１１种限制性内切酶（ＢａｍＨⅠ、ＢｇｌⅠ、ＢｇｌⅡ、ＢｃｏＲⅠ、ＨｐａⅠ、ＫｐｎⅠ、ＰｓｔⅠ、ＳａｃⅠ、ＳａｌⅠ、ＸｈｏⅠ、ＸｂａⅠ）分析鳙鱼的线粒体ＤＮＡ（ｍｉｔｏｃｈｏｎｄｒｉａｌＤＮＡ，简称ｍｔＤＮＡ），构建了全部１１种酶２２个切点的物理图谱．鳙鱼线粒体ＤＮＡ的分子大小约为１６．４４ｋｂ．酶切位点的分布是非随机的．The mtDNA of Aristichthys has been isolated and purified for restriction enzyme analysis. Its length is 16.44 kb,and its molecular weight is 10.17×108 dation. The mtDNA has been analyzed by 11 kinds of restriction endonucleases which all recognized 6 basepairs. The restriction mapof the total 22 cut Sites have been constructed according to the data of molecular weight of generated fragmentS by complete digestion with single and double enzymes,sometimes also with partial digestionof single enzyme. The distribution of endonuclease sites is not even on the mtDNA of this kind of fish.

关 键 词：鳙鱼 线粒体 DNA 限制性内切酶 

分 类 号：Q959.468[生物学—动物学]