结球白菜高效离体子叶不定芽再生的研究  

Studies on high efficient in vitro shoot regeneration from cotyledon of Chinese cabbage

作　　者：樊明琴[1] 朱月林[1] 朱丽华[1] 

机构地区：[1]南京农业大学园艺学院,江苏南京210095

出　　处：《南京农业大学学报》2005年第1期20-23,共4页Journal of Nanjing Agricultural University

基　　金：教育部留学回国人员科研启动基金资助项目 ( [2001] 498)

摘　　要：以2个日本优质结球白菜爱知结球白菜(C1)和新理想白菜(C2)的子叶为外植体,研究了不同激素配比、AgNO3浓度和苗龄对不定芽再生的影响.结果表明,与6-BA相比,TDZ对子叶不定芽再生的效果更好.在MS+0.5 mg·L-1 NAA+1 mg·L-1 TDZ培养基中,2个品种的子叶不定芽再生率分别达到75.0%和55.0%.在此基础上,添加5 mg·L-1 AgNO3时,C1和C2子叶不定芽再生率分别达到96.7%和80.0%,并以5～8 d为子叶不定芽再生的最佳苗龄.The cotyledons were used as explants from two excellent quality Chinese cabbage cultivars,Aichi Hakusai(C 1)and Shinriso Hakusai(C 2),introduced from Japan.Effects of hormone combinations,AgNO 3 concentration and seedling age on shoot regeneration from cotyledon were studied.The results were as follows.Compared with 6-BA,TDZ was more effective in inducing shoot regeneration.The shoot regeneration percentage from cotyledons of the two cultivars reached 75.0% and 55.0% respectively in MS medium containing 0.5 mg·L -1 NAA and 1 mg·L -1 TDZ.Adding 5 mg·L -1 AgNO 3 into the above medium could further increase the frequency of shoot regeneration up to 96.7% and 80.0% respectively,and seedling age from 5 to 8 days was the best.

关 键 词：结球白菜 子叶 不定芽再生 

分 类 号：S634.1[农业科学—蔬菜学] Q813.1[农业科学—园艺学]