美国枫香茎段组织培养与植株再生  

Tissue Culture of Stem Segments and Plantlet Regenation of Liquidambar styraciflua

作　　者：苏梦云[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400

出　　处：《林业科学研究》2005年第1期98-101,共4页Forest Research

基　　金：耐水湿;耐盐碱优良树种资源引进(编号2000 04 15;948项目)

摘　　要：The results obtained showed that browning rate of explants from stem segments with bud scale of seedlings of American sweetgum （Liquidambar styraciflua） collected in April was lower,then it gradually raised after April.Inducing rate of explants callus in April and May were quicker than that in June to Oct.Controlling effects of oxidative browning on WPM medium supplemented with different antioxidant are as follows:ascorbic acid (5 mg·L-1)>mercapto ethanol (10 ml·L-1)>polyvinylpyrrolidone (PVP,1 g·L-1)>active charcoal (1 g·L-1).Oxidative browning rates changed from 27.5% （CK） into 2.5%,15%,20% and 20%,respectively.Inducing rate of calli and adventitious buds on WPM medium supplemented BA （6-Bbenzyl aminopurine） and Kinetin（KT） was higher than that without KT in the medium.A large number of calluses and more adventitious bud of Explants on WPM medium containing 1 mg·L-1 BA,0.5 mg·L-1 KT,0.2 mg·L-1,0.2 mg·L-1 IBA and 5 mg·L-1 ascorbic acid could induce not only a large number of callu （inducing rate:92%） but also more adventitious buds.The medium supplemented with 1 mg·L-1 indole-3-acetic acid （IAA） favored the forming of clumping shoots.Plantlets rooted well on 1/2 MS medium containing 0.2 mg·L-1 naphthalene acetic acid （NAA）, 0.3 mg·L-1 indole butyric acid （IBA）, and 5 g·L-1 active charcoal.The rooting ratio was 75%.The results obtained showed that browning rate of explants from stem segments with bud scale of seedlings of American sweetgum (Liquidambar styraciflua) collected in April was lower,then it gradually raised after April.Inducing rate of explants callus in April and May were quicker than that in June to Oct.Controlling effects of oxidative browning on WPM medium supplemented with different antioxidant are as follows:ascorbic acid (5 mg·L^(-1))>mercapto ethanol (10 ml·L^(-1))>polyvinylpyrrolidone (PVP,1 g·L^(-1))>active charcoal (1 g·L^(-1)).Oxidative browning rates changed from 27.5% (CK) into 2.5%,15%,20% and 20%,respectively.Inducing rate of calli and adventitious buds on WPM medium supplemented BA (6-Bbenzyl aminopurine) and Kinetin(KT) was higher than that without KT in the medium.A large number of calluses and more adventitious bud of Explants on WPM medium containing 1 mg·L^(-1) BA,0.5 mg·L^(-1) KT,0.2 mg·L^(-1),0.2 mg·L^(-1) IBA and 5 mg·L^(-1) ascorbic acid could induce not only a large number of callu (inducing rate:92%) but also more adventitious buds.The medium supplemented with 1 mg·L^(-1) indole-3-acetic acid (IAA) favored the forming of clumping shoots.Plantlets rooted well on 1/2 MS medium containing 0.2 mg·L^(-1) naphthalene acetic acid (NAA), 0.3 mg·L^(-1) indole butyric acid (IBA), and 5 g·L^(-1) active charcoal.The rooting ratio was 75%.

关 键 词：美国枫香 茎段 组织培养 

分 类 号：S722.37[农业科学—林木遗传育种]