山羊胎儿脑室脉络丛发育中NGF及其受体TrKA的表达  

Expression of NGF and TrKA in Developing Choroid Plexus of Goat Fetus

作　　者：卿素珠[1] 徐永平[1] 张涌[1] 

机构地区：[1]西北农林科技大学动物科技学院,杨凌712100

出　　处：《畜牧兽医学报》2005年第3期250-254,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金重点资助项目(39830280)

摘　　要：运用常规组织学技术和免疫组织化学SP法对山羊胎儿脑室脉络丛的组织发生及发育过程中神经生长因子(nerve growth factor ,NGF)和其受体TrKA的表达进行了详细观察,结果显示:山羊胎儿脑室脉络丛最初是由脑泡上皮凸出形成简单的褶、而后形成复杂的分支,上皮由假复层柱状向单层柱状过渡、最后变为单层立方上皮,上皮细胞形态由柱状变为立方、个体逐渐变小,在发育各时期上皮顶端均可见到嗜酸性的刷状缘。在各发育阶段的脑室脉络丛上皮存在NGF及其受体TrKA的规律性表达,反应产物的亚细胞定位略有不同,NGF主要定位于胞核、尤以核膜着色更甚,TrKA则主要定位于核膜。脉络丛上皮内源性 NGF的功能可能涉及两方面,一是对脑室脉络丛上皮细胞的发育成熟起营养作用;另一方面分泌产物可进入脑脊液并通过脑脊液循环对脑的发育发挥调节作用。The histogenesis of choroid plexuses in goat brain ventricle were observed by using histological technology,and immunohistochemical ultra sensitive SP method has been used to examine the expression of nerve growth factor (NGF) and its high-affinity receptor (TrKA) in developing choroid plexuses simultaneously.The results suggested that during embryonic development,the choroidal epithelium lining the brain ventricle transited from psedostratified columnar to simple columnar, and became simple cuboidal layer at last.The epithelial cell changed from column to cube in shape,from big to small in diameter.Well-developed acidophilic brush border could be observed at the top of epithelium during development.It was established that during the period studied choroidal epithelium of NGF and TrKA positive reactivity were present.There were little differences between cellular localizaton of two markers,NGF positive products were mainly occurred on nucleus,especially on nucleus membrane,but TrKA positive products were mainly on nucleus membrane.Endogenous NGF in choroidal epithelium suggested major roles in development and maturation of itself,and also in development of brain by circulation of blood-cerebrospinal fluid.

关 键 词：脑室 NGF 上皮 脉络丛 羊胎 受体 表达 山羊 发育 育成 

分 类 号：S852.659.3[农业科学—基础兽医学] R743[农业科学—兽医学]