用ALV-Jgp85单克隆抗体证明蛋鸡存在J亚群禽白血病  

Occurrence of ALV-J in Egg-type Chickens Certified by Monoclonal Antibody against ALV-J gp85

作　　者：徐镔蕊[1] 董卫星[2] 余春明[1] 冯小宇[1] 李宁[1] Lucy F.Lee Maoxiang Li 

机构地区：[1]中国农业大学动物医学院,北京100094 [2]安徽省农业委员会畜牧局,合肥230001 [3]AvianDiseaseandOncologyLab.USDA.EastLansing

出　　处：《畜牧兽医学报》2005年第3期269-271,共3页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30371073);北京市自然科学基金(5053027)

摘　　要：采用免疫组化法,对病理学初步诊断为蛋用型鸡 J亚群白血病的自然发病鸡的肿瘤、骨髓、肝脏、脾脏、肾脏、肺脏、心脏、胰脏、输卵管、卵巢、腺胃、骨骼肌、大脑、坐骨神经,用特异性抗 J亚群禽白血病病毒(ALV J)囊膜糖蛋白gp85的单克隆抗体进行检测,待检的组织切片中均检出阳性抗原,免疫组化的研究结果与病理学诊断结果相一致。在国内外首次发现并报道蛋用型鸡 J亚群禽白血病的自然病例。Immunohistochemical method was used to detect avian leukosis virus subgroup J from egg-type chicken, which was preliminarily diagnosed to appear subgroup J avian leukosis. The organs from these chickens were examined by ALV-J gp85 monoclonal antibody, these organs included marrow, liver, spleen, kidney, lung, heart, pancreas, oviduct, ovary, ventriculus glanduaris, skeletal muscle, cerebrum, ischiacticus nerve. The results from immunohistochemistry using monoclonal antibody against gp85 envelope protein of avian leukosis virus subgroup J(ALV-J) revealed that all examined organs contained detectable antigen of ALV-J. The result of immunohistrochemistry agrees with that of pathology diagnosis. It is the first study at home and abroad that discovered and reported natural disease of ALV-J in egg-type chickens.

关 键 词：单克隆抗体 诊断 肿瘤 首次发现 膜糖蛋白 检出 免疫组化法 J亚群禽白血病 ALV-J 蛋用型 

分 类 号：S858.31[农业科学—临床兽医学] S852[农业科学—兽医学]