伪狂犬病基因缺失疫苗株(SA215)生物学特性研究  

The Biological Characteristics of a Gene-deleted Pseudorabies Virus Vaccine Strain (SA215)

作　　者：陈陆[1] 郭万柱[1] 徐志文[1] 王小玉[1] 王印[1] 

机构地区：[1]四川农业大学动物生物技术中心

出　　处：《畜牧兽医学报》2005年第3期278-282,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(39570544);国家"九五"科技攻关项目(96 C01 04 03)

摘　　要：测定了伪狂犬病gE-/gI-/ TK-/ LacZ+基因缺失疫苗株(SA215)的致细胞病变效应、安全性、免疫原性和接种动物抗体消长规律等生物学特性。试验结果显示,该疫苗株能在 Vero细胞上适应生长,并形成典型的蚀斑。其对1日龄仔猪、妊娠母猪、牛、羊以及家兔安全,无不良接种反应,接种动物不向外散毒。SA215 疫苗接种猪能抵御高剂量(107 PFU) Fa株强毒感染,攻毒后试验猪的发热期、增重受阻天数、散毒滴度略低于 Bartha株疫苗接种猪,低于对照组猪。SA215接种猪能维持长时间的高水平中和抗体滴度。试验结果表明,SA215 株是一株安全、免疫原性好的疫苗株。The cell culture character, safety, efficacy and antibody duration of gene-deleted Pseudorabies virus vaccine strain (SA215), which lacked gE, gI and thymidine kinase (TK) genes, but can express LacZ gene were tested in this study. SA215 could adapt to Vero cells and induce plaque formation. It was safe to 1-day-old piglets, pregnant sows, cattle, sheep, goats and rabbits, moreover, the animals vaccinated with SA215 strain were less clinically affected and the SA215 strain virus couldn’t be recovered from vaccinated animals. Inoculation of SA215 could protected pigs from challenge of Fa strain,the virulent Pseudorabies virus, at a dose of 10~7 PFU. The periods of fever and growth arrest and titer of excreting virus of the pigs vaccinated with SA215 were lower than those of the pigs vaccinated with Bartha strain, but were much lower than that of the unvaccinated pigs. The vaccinated pigs with SA215 strain produced high-level anti-PRV neutralizating antibodies that could provide protection from infection till 16 weeks after vaccination. The results showed that SA215 strain was a safe vaccine that had good immunogenicity.

关 键 词：基因缺失疫苗 伪狂犬病 种猪 接种 生物学特性研究 强毒感染 增重 SA 滴度 疫苗株 

分 类 号：S858[农业科学—临床兽医学] S828[农业科学—兽医学]