川楝素对昆明小鼠的胚胎毒性研究  

Research of Embryotoxicity of Toosendanin in Kunming Mice

作　　者：张先福[1] 王建华[1] 张树方 刘颖[3] 樊立超 史夏云 

机构地区：[1]西北农林科技大学动物科技学院,杨凌712100 [2]山西省家畜疫病防治站,太原030024 [3]山西大同医学专科学校,大同037000

出　　处：《畜牧兽医学报》2005年第3期301-305,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30070577)

摘　　要：取体重18～25 g雌鼠,超数排卵后与公鼠合笼.获得的孕鼠随机分为6组,分别用于研究川楝素对孕鼠2-细胞胚、桑椹胚、囊胚3个时期胚胎的毒性.每个时期均设对照组,以小鼠腹腔注射1/30半数致死量(LD50)的川楝素剂量对以上3个时期试验组孕鼠染毒2次,对照组孕鼠用等量PBS注射.研究其着床时期胚胎毒性时不对小鼠进行超数排卵,但试验组孕鼠染毒3次,对照组孕鼠用等量PBS注射.最后计数、观察4个时期孕鼠子宫内的胚胎数量和形态.结果表明:2-细胞胚、桑椹胚、囊胚时期试验组胚胎总数与对照组相比,没有统计学上差异(P＞0.05);2-细胞期试验组正常胚胎、异常胚胎数量与对照组相比,也无统计学上的差异(P＞0.05);桑椹胚、囊胚时期试验组正常胚胎、异常胚胎数量与对照组相比,具有极显著差异(P＜0.01).3次染毒对着床后的胚胎毒性最大,子宫内胚胎几乎全部溶解,无法计数.母体组织病理切片观察发现川楝素对主要器官心、肝、肺、肾、子宫仅有轻微损害,提示腹腔注射小剂量川楝素对怀孕小鼠具有特定的胚胎毒性.Virgin female mice weighting 18～25g were superovulated, and then mated with male mice. The acquired pregnant mice were divided into 6 groups for embryotoxicity research of toosendanin on embryo of mice in 2-cell, morula, blastocysts stages. Control group was set up in each of these three stages. Every pregnant mouse of treated and control groups in the three stages was given the dose of 1/30 LD_(50) toosendanin and same amount of PBS 2 times by intraperitoneal injection respectively. Superovulation need not be carried out for embryotoxicity study of toosendanin in implantation stage, but each pregnant mice in treated and control group was given the dose of 1/30 LD_(50) toosendanin and PBS 3 times by intraperitoneal injection respectively. Finally, the number and configuration of embryo in uteri of all groups were counted and examined. Results indicated that total embryo of treated group in 2-cell, morula and blastocysts stages had no significance difference comparing with corresponding control group (P> 0.05); Number of normal and abnormal embryo of treated group in 2-cell stage had no significance difference comparing with control group either (P> 0.05); Number of normal and abnormal embryo of treated group in morula and blastocysts stages had great significance difference comparing with corresponding control group (P< 0.01). Due to injection to the treated group 3 times in implantation stage, almost all the fetuses in uteri had been melting, and counting of them could not be performed, while healthy fetuses could be seen and counted clearly in control group. There were only mild histological changes founded in maternal heart, liver, lung, kidney and uteri in all treated groups. All above means that the intraperitoneal injection of a small amount of toosendanin to pregnant Kunming mice may has special embryotoxicity.

关 键 词：孕鼠 对照组 胚胎毒性 川楝素 染毒 小鼠 宫内 桑椹胚 时期 超数排卵 

分 类 号：S826.36[农业科学—畜牧学] R321[农业科学—畜牧兽医]