微柱高效液相色谱法测定岩白菜素含量研究  

Study on Microcolumn High Performance Liquid Chromatography for the Determination of Bergeninum in Rodgersia Pinnata Hemsl and Compound Bergeninum Tablets

作　　者：张甜[1] 郝南明[2] 董学畅[1] 张云霁 

机构地区：[1]云南民族大学化学与生物技术学院,云南昆明650031 [2]文山师范高等专科学校,云南文山663000 [3]云南省施普瑞生物工程有限公司,云南昆明650106

出　　处：《云南民族大学学报（自然科学版）》2005年第2期128-130,共3页Journal of Yunnan Minzu University:Natural Sciences Edition

摘　　要：　研究了用微柱高效液相色谱法测定岩陀及复方岩白菜素片中岩白菜素的含量.样品中岩白菜素用甲醇超声振荡提取,提取液通过Sep-Pak-C18固相萃取小柱过滤收集,然后以WatersXterraTMRP18(1.0×50mm,2.5μm)微柱为固定相,22%的甲醇为流动相分离,岩白菜素在0.99min即可达到基线分离,用紫外二极管矩阵检测器检测.方法标准回收率为98.1%～104%,相对标准偏差为1.7%～2.2%.此方法可快速、准确的测定岩白菜样品中岩白菜素的含量.A microcolumn high performance liquid chromatography method for the determinationof bergeninum in Rodgersia pinnata Hemsl and compound bergeninum tablets was studied. The bergeninum was extracted from the sample with methanol by ultrasonic extraction for 45 min. Then the bergeninum was separated on a Waters Xterra^(TM) RP_(18)(1.0×50mm, 2.5μm) chromatogram microcolumn with 22% methanol in 0.99 min, and monitored with the photodiode array detector. The recoveries of the flavonoids are 98.1%～104%, and relative standard deviations are 1.7%～2.2%. This method was applied to the determination of bergeninum with good results.

关 键 词：微柱高效液相色谱 岩白菜素 分析 

分 类 号：O652.63[理学—分析化学]