条斑紫菜Porphyra yezoensis Ueda营养细胞的分离和培养  

Isolation and Cultivation of the Vegetative Cells of Porphyra Yezoensis Ueda

作　　者：赵焕登[1] 张学成[1] 

机构地区：[1]山东海洋学院海洋生物学系

出　　处：《山东海洋学院学报》1981年第1期61-66,共6页

摘　　要：条斑紫菜营养细胞在一定条件下(主要是温度)可转化成单孢子，经放散、附着，再发育成新的藻体。但单个营养细胞在未形成单孢子以前，能否发育成新藻体尚未见报导。1,Individual vegetative cells of Porphyra yezoensis were obtained by mechanical smashing and filtering. After isolation, cultioation, the cell wall of the isolated cells began to become thickened and the cells each began to divide and developed into a new frond. The vegetative cells of Porphyra yezoensis have been observed to be totipotent in development. The furvival rate of the isolated cells was about 33%.2, The isolated cells could develop into new fronds under4 °-22℃, but the optimal temperature was found to be 12°-13℃.3 , Special supplementary culture and continuous illumination seemed to be able to quicken the developing process.

关 键 词：条斑紫菜 营养细胞 单孢子 分离方法 培养条件 

分 类 号：S968.431[农业科学—水产养殖]