柱孢鱼腥藻的铁蛋白与棕色固氮菌的钼铁蛋白的交叉互补作用  

CROSS-REACTIVITY OF Fe-PROTEIN OF ANABAENA CYLINDRICA AND MoFe-PROTEIN OF AZOTOBACTER VINELANDⅡ

作　　者：何振荣[1] 林惠民[1] 杜代贤[1] 戴玲芬[1] 辛武生[1] 黎尚豪[1] 

机构地区：[1]中国科学院水生生物研究所

出　　处：《水生生物学集刊》1985年第3期241-247,共7页

摘　　要：纯化的柱孢鱼腥藻铁蛋白能够与棕色固氮菌的钼铁蛋白有效地交叉反应,展现较高的活性。此异源交叉反应的乙炔还原比活及放氢比活,分别是蓝藻同源互补比活的83.8及66.7％。比较藻铁蛋白与菌钼铁蛋白异源交叉反应及藻固氮酶组分之间的同源反应的动力学特点时发现,铁蛋白对钼铁蛋白的最佳克分子比数前者(异源交叉反应)较后者(藻同源反应)为高,前者为5,后者为1;但反应的时间进程两者差别不大。A modified method was used for the separation and purification of Anabaena cylindrica nitrogenase, based upon which a further study on the cross-reactivity between Fe-protein from A. cylindrica and MoFe-protein from Azotobacter vinelandii was made. Results indicated that these two heterologous components were able to give an effective cross-reaction and showed a relatively strong nitrogenase activity. The specific activities of acetylene reduction and H_2-evolution contributed by cross-reacting heterologous components were as high as 83.8% and 66.7%, respectively, of that produced by algal homologous complementary response. In comparison with the kinetics of the algal homologous complementary reaction, it was found that the optimum molar ratio of Fe-protein to MoFe-protein for heterologous cross-reaction is much higher (5:1), the time course for tooth reactions being approximately the same. Discordant with the current conception, our findings demonstrate that the Fe-protein of A. cylindrica can form effective complexes with the MoFe-protem of Azotobacter.

关 键 词：柱孢鱼腥藻 铁蛋白 棕色固氮菌 钼铁蛋白 交叉互补作用 固氮酶 

分 类 号：Q949.2[生物学—植物学] Q945.13