草鱼出血病潜伏期和发展期的血液病理研究  

HAEMOPATHOLOGICAL STUDY ON LATENT AND DEVELOPING PERIOD OF HEMORRHAGIC DISEASE OF GRASS CARP FINGERLINGS

作　　者：朱心玲[1] 贾丽珠[1] 张明瑛[1] 

机构地区：[1]中国科学院水生生物研究所

出　　处：《水生生物学报》1987年第1期59-66,共8页Acta Hydrobiologica Sinica

摘　　要：草鱼种经人工感染呼肠孤病毒后进行了血液学和血液化学的检查。红血球、血浆总蛋白和尿素氮在感染4天的潜伏期鱼体内,发现显著的减少。当进入发展期(第8天),红血球、血红蛋白、血浆总蛋白和尿素氮的含量减少更明显。SLDH同工酶谱的相对活性在潜伏期和发展期病鱼的血清内出现紊乱现象,同时,在某些感染鱼的血清中,明显可见一条多出的区带。此病流行期间,早期的血液变化已经发生,某些生理变化已在红血球、血红蛋白、血浆总蛋白和尿素氮中得到反映,其含量的变化与人工感染组的结果相似。所测的血清钾含量明显增加;血清钙的含量明显减少。值得提出的是血红蛋白、红血球、血清钙和钾等被证实有早期变化。The paper describes some of the early changes in blood of the grass carp following artificial infection of Reovirus. The activities of serum lactic dehydrogenase (SLDH) isozyme of Reovirusinfected fish were compared with normal individuals. Hematological changes of fish naturally suffering from this disease were studied at the epizootic area. All data obtained were treated statistically.Hematological and blood chemical changes were examined after fish were infected artificially by Reovirus. Significant reduction of red blood cell(RBC), total plasma protein and urea nitrogen occurred in the latent period by 4—5 days, although there were no obvious symptoms. When entering the developing period by 8 days, fish were moribund with typical hemorrhagic symptom, with RBC, hemoglobin (Hb) and total protein reduced even more obviously. However the urea nitrogen of the diseased fish was still maintained at the level of latent period. The plasma glucose concentration also changed insignificantly. The relative activity of SLDH isozyme seemed to be deranged during the latent and developing periods of the disease. In the control fish, its order was SLDH_1<SLDH_2<SLDH_3<SLDH_4<SLDH_5 but in the infected fish, the order was reversed into SLDH_1>SLDH_2>SLDH_3>SLDH_5>SLDH_4>SLDH_6, in which the last band (SLDH_6) was detected near negative pole on the cellulose acetate membrane electropherogram of some infected fish.The early hemopathologic changes in fish occurred during epizootic period. Two groups of fish were examined. One group consisted of fish with pronounced symptom, and the other group was taken randomly from the same fish pond. Results showed that changes in RBC, Hb, total protein, glucose concentration, urea nitrogen and SLDH isozyme occurred in both groups and were similar to those found in artificially infected fish. Moreover, there was a marked increase in the level of serum potassium and an obvious reduction of serum calcium. Correlation between body weight of infected fish in the latent period and various blood pa

关 键 词：潜伏期 红血球 尿素氮 血红蛋白 血浆总蛋白 血清钙 含量 人工感染 草鱼出血病 血液变化 

分 类 号：Q786[生物学—分子生物学] S858[农业科学—临床兽医学]