β-二酮型高分子纳米微球与核酸相互作用的共振光散射光谱及分析应用  

The Resonance Light Scattering Spectra from the Interact ion of Zn-PVK Nanoparticles with Nucleic Acids and Its Analytical Application

作　　者：周运友[1] 佘世科[1] 章丽[1] 卢琴[1] 赵成安[1] 王伦[1] 

机构地区：[1]安徽师范大学化学与材料科学学院,安徽省芜湖市241000

出　　处：《光谱实验室》2005年第1期25-29,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金 (2 0 375 0 0 1)资助项目

摘　　要：在超声辐射下 ,过氧化氢氧化降解聚乙烯醇 (PVA) ,制得与 Zn2 +形成配位体的纳米尺寸的 β-二酮型高分子微球 (Zn- PVK)。对 Zn- PVK的共振光散射 (RLS)性质研究发现 ,Zn- PVK与核酸形成缔合物时将导致 Zn- PVK的 RLS信号急剧增加 ,据此建立一种用 RLS信号测定痕量核酸的新方法。方法的抗干扰能力强 ,用于合成样品的分析 ,结果令人满意。A kind of functional zinc-β-diketone complex nanoparticle(Zn-PVK)was pre pared by an oxidation and degradation under ultrasonic radiation,and characteriz ed as spherical with the diameters from 20 to 50 nm by TEM.The interaction of Zn -PVK with nucleic acids results in a great enhancement of resonance light scatt ering(RLS).A new method for the determination of trace nucleic acids was establi shed based on the intensity enhancement of RLS under the optimium conditions.Thi s method can tolerate the interference of various substances such as various met al ions,amino acids,proteins,and surfactant,and can be applied to analyze the sy nthetic samples with satisfactory results.

关 键 词：Β-二酮 高分子微球 超声辐射 共振光散射光谱 分析应用 缔合物 纳米微球 核酸 RLS 相互作用 

分 类 号：O614[理学—无机化学] O657[理学—化学]