蒽环类抗肿瘤抗生素与DNA作用差异性的光谱电化学研究  

Study on the Difference between Several Anthracycline Antibiotics' Action with DNA by Spectroelectrochemistry

作　　者：丁敏[1] 程圭芳[1] 赵洁[1] 何品刚[1] 方禹之[1] 

机构地区：[1]华东师范大学化学系,上海200062

出　　处：《化学通报》2005年第3期198-203,197,共7页Chemistry

基　　金：国家自然科学基金资助项目 (2 9875 0 0 8)

摘　　要：利用在线紫外 可见光谱电化学技术、微分脉冲伏安法及琼脂糖凝胶电泳技术考察了柔红霉素、表阿霉素、阿克拉霉素、伊达比星与DNA的作用。实验表明 ,药物与DNA形成复合物的稳定性主要取决于药物的糖环与DNA之间的作用 ,糖苷配基上取代基与DNA形成氢键也有一定影响。另外 ,DNR在还原代谢过程中最易产生半醌自由基。The interaction of daunorubicin, aclacinomycin-A, epirubicin and idarubicin with DNA has been studied by UV-spectroelectrochemistry and differential pulse voltammetry methods, in combination with agarose gel electrophoresis. The results show that the chemical structure of sugar rings take precedence over chemical modifications of the aglycone moiety with regard to the drug-DNA binding constant. It is the easiest to generate the free radical for DNR than the other three ant ibiotics.

关 键 词：DNA 蒽环类 抗肿瘤抗生素 柔红霉素 表阿霉素 阿克拉霉素 作用 光谱电化学 取代基 氢键 

分 类 号：R96[医药卫生—药理学]