安氟醚麻醉大鼠不同时期脑Ca^(2+)-ATP酶活性变化  

Enflurane on Ca^(2+)-ATPase activity change in rats

作　　者：李元涛[1] 戴体俊[2] 曾因明[2] 朱涛[1] 姚尚龙[3] 刘菊英[1] 

机构地区：[1]湖北省十堰市郧阳医学院麻醉学系附属太和医院麻醉科,442000 [2]徐州医学院江苏省麻醉学重点实验室 [3]武汉市华中科技大学协和医院麻醉科

出　　处：《临床麻醉学杂志》2005年第3期191-192,共2页Journal of Clinical Anesthesiology

基　　金：国家自然科学基金资助课题(No39970715);江苏省自然科学基金资助课题(NoBK2001143)

摘　　要：目的　动态观察安氟醚吸入麻醉大鼠不同时期各脑区Ca2+ ATP酶活性变化和行为学 变化,探讨安氟醚的麻醉作用机制。方法　40只SD雌性大鼠随机均分为五组:诱导期组、麻醉期组、 恢复期组、清醒期组和对照组。分光光度法测定不同时期大脑皮层、脑干、海马Ca2+ ATP酶活性。结 果　在诱导期各脑区Ca2+ ATP酶活性即开始下降(P<0.05);麻醉期降至最低(P<0.01);恢复期又 开始升高,但仍低于对照组水平。其中,仅脑干Ca2+ ATP酶活性降低有显著性差异(P<0.05);清醒 期基本恢复至对照组水平(P>0.05)。且行为学变化与脑Ca2+ ATP酶活性变化有关。结论　安氟醚 的麻醉效应可能与其抑制脑Ca2+ ATP酶活性相关,麻醉深浅与抑制的程度一致。Objective To observe a dynamic effect of enflurane on the Ca 2+-ATPase activity in different brain region at different anesthesia stages,and explore the anesthetic mechanism of enflurane.Methods Forty femal rats were divided randomly into five groups of control group,induction group,anesthetic group,recovery group,and awake group. The Ca 2+-ATPase activity in the cerebral cortex, brain stem and hippocampus were measured by spectroscopic analysis at different anesthesia stages.Results The activity of Ca 2+-ATPase in different brain regions begun to reduce during the induction stage(P<0.05),and reached to the lowest during anaesthetic stage,and begun to increase during recovery stage but still lower than that in nomal group.Only the decrease in the activity of Ca 2+-ATPase in the brain stem was significant(P<0.05),which recovered almost to the nomal level during awake stage(P>0.05). The praxiology change was also related to the change of Ca 2+-ATPase activity.Conclusion Enflurane could work through inhibiting the activity of brain Ca 2+-ATPase, and the depth of anesththesia was related to the degree of the inhibition.

关 键 词：安氟醚 麻醉 脑 CA^2+-ATP酶 活性变化 

分 类 号：R614[医药卫生—麻醉学]