高效液相色谱法测定1－硝基蒽醌和1－氨基蒽醌  

DETERMINATION OF 1-NITROANTHRAQUINONE AND 1-AMINOANTHRAQUINONE BY THE HICH-PERFORMANCE LIQUID CHROMATOGRAPHY

作　　者：黄明宪[1] 李红莉[1] 张正 

机构地区：[1]西南师范大学化系

出　　处：《西南师范大学学报（自然科学版）》1994年第3期271-275,共5页Journal of Southwest China Normal University(Natural Science Edition)

摘　　要：探讨了１－硝基蒽醌（１－ＮＯ＿２ＡＱ）和１－氨基蒽醌（１－ＮＨ＿２ＡＱ）同时测定和分别测定的色谱条件，提出了在Ｓｈｉｍ－ｐａｃｋＣＬＳＳ－ＳＩＬ色谱柱上，以正庚烷－１，４二氧六环－乙酸乙酯（６５：３５：５（Ｖ／Ｖ）为流动相，流量为１．５ｍｌ／ｍｉｎ，柱温４０℃，以分光光度检测器于２５４ｎｍ处检测时，４ｍｉｎ内使１－ＮＯ＿２ＡＱ与１－ＮＨ＿２ＡＱ分离；５ｍｉｎ使１－ＮＨ＿２ＡＱ与杂质分离；７ｍｉｎ可使１－ＮＯ＿２ＡＱ与杂质分离的高效液相色谱分析法。其绝对检出下限１－ＮＯ＿２ＡＱ为０．０１２μｇ，１－ＮＨ＿２ＡＱ为０．０２６μｇ，变异系数分别为１．１％和０．９６％，回收率分别为９９．７％和１００．２％。The chromatography conditions for simultaneous determination of 1-NO_2AQ and 1-NH_2AQ were presented,It was suggested that in a HPLC with the column of Shim-pock CLC-SIL,the best conditionswere as follows:a n-heptane-1,4-dioxacyclohexane-ethylacetate(65:35:5 V/V)as mobile phasewith flow rate 1.5 ml/min and column temparature 40℃,as well as with detector monitoring at 254nm. In the above conditions 1-NO_2AQ can be separated from 1-NH_2AQin mins., 1-NH_2AQ from im-purity in 5 mins,,and1-NO_2AQ from impurity in 7 mins. The absolute limits are 0.12μg for 1-NO_2AQ and 0.026μg for 1-NH_2AQ,The variation coefficients are 1.1%and 0. 96%respectively,while the recvery rates are 99.7%and 100.2%respectively.

关 键 词：硝基蒽醌 氨基蒽醌 高效液相色谱 

分 类 号：O625.463[理学—有机化学]