用M13DNA探针检测小鼠DNA指纹  

The Defection of DNA Fingerprints with M13 phage DNA Probe in the Mouse

作　　者：罗鹏[1] 罗林[1] 梁松 

机构地区：[1]西南民族学院畜牧兽医系,四川大学生物工程系,四川省寄生虫研究所

出　　处：《西南民族学院学报（自然科学版）》1994年第3期289-292,共4页Journal of Southwest Nationalities College(Natural Science Edition)

摘　　要：用ＨｉｎｆＩ酶切，随机引物法标记的Ｍ１３ｍｐ１８ＤＮＡ作探针，对无关小鼠个体及一个小鼠家系三代进行了ＤＮＡ指纹分析，无关个体平均每个个体得到１４条谱带（＞４ｋｂ）。无关个体具有相同指纹图概率为９×１０￣（－８），显示高度个体材异性，对小鼠家系内后代与亲本图谱进行了比较，表明图谱特征按孟德尔方式遗传。对家系内个体间遗传相似度Ｄ进行了计算，亲缘关系近则Ｄ值高，据此对本技术在动物个体识别，亲缘关系分析等方面的应用进行了讨论。Hypervairable polymorphic patterns are detected with Hinf I digestion and phage M13DNA as a probe in genomic DNA of Mice,14 minisatellite-containing DNA fragments are observed perindividual.The probability that two unrelated indivduals have identical pattern is 9 ×10￣(-8) ,showingthat the DNA fingerprints are individual specific,Comparison of the bending pettern of offsprings withtheir parents in a known mouse’s family reveals that patterns are inherited as stable traits. The valuesof D(Genetic similarity ) are calculated among the individuals in the mouse’ s family, Ds are higherwhen the genetic relationship is closer.The application of this technique in animal genetic analysis,suchas individual indentification , peternity teeting and strain analysis ,are also disacused.

关 键 词：DNA指纹 M13噬菌体 小鼠 遗传分析 

分 类 号：Q953[生物学—动物学]