超临界CO_2流体萃取延胡索中延胡索乙素的正交试验研究  

Supercritical CO_2 Fluid Extraction of Tetrahydropalmatine from Corydalis yanhusuo W.T.Wang by Orthogonal Design

作　　者：陈峰[1] 郭立玮[1] 井山林[1] 王玳珠[2] 

机构地区：[1]南京中医药大学植物药深加工工程研究中心,南京210029 [2]苏州市中医院,江苏苏州215003

出　　处：《中药新药与临床药理》2005年第2期137-138,152,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：江苏省高校高新技术产业化项目(编号JH02-104);苏州市科技发展计划项目(编号SS0102)

摘　　要：目的探索延胡索中延胡索乙素超临界CO2流体萃取(SFE-CO2)的最佳工艺条件。方法以延胡索乙素转移率为指标,考察超临界CO2流体压力、温度、夹带剂用量对萃取效果的影响。结果最佳工艺条件为:压力15Mpa,温度40℃,夹带剂为药材1.5倍量的95%乙醇。结论该工艺稳定可行,具有操作温度低、耗能低、时间短、有效成分含量高、无有机残留等优点。Objective To find out the optimal supercritical CO2 fluid extr ac tion prosess for Tetrahydropalmatine. Methods The extraction process was optimiz ed by orthogonal design with the yield of Tetrahydropalmatine as markers. Three factors including extraction pressure, temperature and the volume of entrainer were observed. Results The optimal condition of supercritical CO2 fluid extrac tion process was: under the pressure of 15Mpa at 40 ℃ , 1.5 fold of 95 % EtO H with Corydalis yanhusuo W.T.Wang as the entrainer. Conclusion The optimal supe rcritical process is feasible, with the advantage of low temperature and energy consumption, short time, high production and no organic residua.

关 键 词：延胡索 SFE—CO2 正交设计 延胡索乙素 HPLC 

分 类 号：R284.2[医药卫生—中药学]