应用旋转组合设计研究C、N、P对苏云金杆菌的影响  

Optimization of growth medium for Bacillus thuringiensis using rotational-combinational design

作　　者：陈聪[1] 李今煜[1] 关雄1 

机构地区：[1]福建农林大学生物农药与化学生物学教育部重点实验室

出　　处：《福建农林大学学报（自然科学版）》2005年第1期34-36,共3页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家"八六三"计划项目(2002AA245011);福建省科技厅重大资助项目(2002N004).

摘　　要：采用二次回归旋转组合设计方法,对苏云金杆菌WB7菌株的溶解性葡萄糖培养基配方进行了摇瓶优化筛选.建立了产孢量与C源、N源、P源的数学模型,分析了各因素的单因素效应和互作效应.试验获得的C、N、P三因素最佳组合为:C源、N源、P源的质量分数分别为 1. 27%、2. 144%、0. 122%.An quadratic-regression-rotational-combinational design was used to screen fermentative medium of Bacillus thuringiensis (Bt) isolate WB7. The regression equation was established which expresses the number of spores of Bt as function, and carbon source, nitrogen source and phosphorus source as variable respectively. Using the regression equation, the single factor effect and mutual effect was studied. The most appropriate fermentative medium is composed of carbon source 1.27%, nitrogen source (2.144%) and phosphorus source 0.122%.

关 键 词：苏云金杆菌 旋转组合 产孢量 二次回归 互作效应 培养基配方 优化筛选 单因素 影响 葡萄糖 

分 类 号：S513[农业科学—作物学] S435