银杏幼胚离体培养再生植株的研究  

Plant Regeneration from Immature Embryos of Ginkgo biloba

作　　者：郭长禄[1] 陈力耕[1] 何新华[1] Syed Asgar Tori 

机构地区：[1]浙江大学农业部园艺植物生长发育与生物技术重点开放实验室

出　　处：《园艺学报》2005年第1期105-107,共3页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30070634)

摘　　要：以佛手银杏幼胚为外植体,研究了不同大小幼胚在不同培养条件下,愈伤组织和胚状体的诱 导发育情况。结果表明:大于3mm的幼胚,在MK+NAA1.0mg·L-1+BA1.0mg·L-1培养基上胚状体 诱导率最高,达到53.6%,最多的一块愈伤组织形成多达38个胚状体。暗培养不利于胚状体的发生。在 MK培养基上添加10%椰汁对胚状体的生长发育有很好的促进作用,有34.5%生长成苗。Factors influencing the induction of callus and somatic embryogenesis were studied with immature embryos of Ginkgo biloba. Highest rate(53.6%)of somatic embryogenesis was obtained on MK medium containing NAA 1.0 mg·L -1+BA 1.0 mg·L -1.Maximum number of embryoids observed on callus was 38 with 3 mm plus embryos and 34.5% embryoids could be developed into plants.Darkness is not suitable for somatic embryogenesis.The growth and development of somatic embryos were promoted by adding 10% CM into the medium.

关 键 词：银杏 胚培养 胚状体发生 

分 类 号：S664.3[农业科学—果树学]