外源Ca^(2+)和ABA对高温下黄瓜花药中Ca^(2+)ABA及蛋白质合成的影响  

Effect of Exogenous Ca^(2+) and ABA on Ca^(2+) Distribution, ABA Contents and Proteins Synthesis in Cucumber Anthers under Heat Stress

作　　者：缪旻珉[1] 朱月林[2] 张玉华[1] 

机构地区：[1]扬州大学农学院园艺系,扬州225009 [2]南京农业大学园艺学院,南京210095

出　　处：《园艺学报》2005年第1期111-114,共4页Acta Horticulturae Sinica

基　　金：国家自然科学基金资助项目(30100123)

摘　　要：外源Ca2+能降低高温下黄瓜单核期花药的胞内Ca2+水平并提高花粉萌发率,而ABA的作用 与此相反。外源Ca2+和ABA处理能显著提高高温下黄瓜花药内源ABA的水平,并产生新的蛋白质。以上 结果表明,高温下外源Ca2+可影响黄瓜花药内ABA信号系统,继而可能改变基因表达,并获得耐热性; 外源ABA亦能影响黄瓜花药内Ca2+信号系统。Exogenous Ca 2+ reduce the intercellular Ca 2+ of cucumber anthers and increase pollen germination percentage at high temperature, while the exogenous ABA have a reverse effect. Endogenous ABA contents in two line anthers ascend significantly linked with new protein formation after exogenous Ca 2+ and ABA treatment under 45℃. The results indicated that Ca 2+ and ABA signals can interact each other in cucumber uninucleate pollen stage anthers under high stress.

关 键 词：黄瓜 高温 花药 钙 ABA 蛋白质双向电泳 

分 类 号：S642.2[农业科学—蔬菜学]