大鼠脊髓半横断损伤对脊髓Ⅱ板层SP表达的影响  

Changes in Expression of SP in Spinal Lamina Ⅱ Following Hemisected Spinal Cord Injury in Mice

作　　者：赵秀军[1] 章为[1] 王廷华[2] 张连双[1] 徐新运[1] 周雪[1] 

机构地区：[1]四川大学华西基础医学与法医学院组织胚胎学与神经生物学教研室,成都610041 [2]昆明医学院神经科学研究所

出　　处：《四川大学学报（医学版）》2005年第2期184-186,共3页Journal of Sichuan University(Medical Sciences)

基　　金：纽约中华医学基金会 (CMB0 0 -72 2 )资助

摘　　要：目的　探讨脊髓半横断对脊髓 板层 SP表达的影响。方法　 2 0只成年 SD大鼠随机分为正常对照组 ,术后 3d、7d、2 1d组。建立成年 SD大鼠脊髓半横断 (T1 3～ L1 )模型。取各组尾侧 L3节段脊髓作冰冻切片。通过免疫组化 ABC法观察 SP免疫阳性反应物的分布 ,计数各组脊髓两侧 板层内 SP免疫阳性神经膨体数。结果　SP免疫阳性产物以神经膨体形式集中分布在脊髓背角 板层内。脊髓半横断后 3d,手术侧脊髓 板层内 SP阳性膨体数较正常组明显增多 ,7d组较 3d组开始下降 ,2 1d恢复正常。非手术侧仅表现为术后 3d一过性增高 ,且反应强度较手术侧有增强。结论　脊髓半横断损伤导致 板层 SP免疫阳性膨体数量改变 ,提示 SP作为伤害感受的一种神经递质或调质可能在脊髓半横断损伤过程中发挥作用。Objective To explore the changes in expression of Substance P(SP) in spinal lamina Ⅱ at different time following hemisected spinal cord injury (hSCI). Methods Twenty Sprague-Dawley rats were randomly divided into intact group and hSCI group at days 3,7 and 21. The spinal cords were hemisected between T 13 and L 1. The animals were sacrificed 3,7,21 days after operation. The L 3 segments were taken out and sectioned continuously into sections (20 μm). The expression of SP was measured by immunohistochemical ABC method, and the number of SP positive varicosities in lamina Ⅱ was counted. Results SP positive varicosities were observed in spinal lamina Ⅱ. Compared with control group, the number of SP positive varicosities on the injured side apparently increased 3 days after hSCI, but decreased 7 days after operation, then recovered to normal by 21 days after operation. Noteworthily, the number of SP positive varicosities on the intact side was higher than that on the injured side. Conclusion SP, as a neurotransmitter of nociceptive information, may be related to the process of hSCI.

关 键 词：SP 神经膨体 脊髓半横断 脊髓Ⅱ板层 

分 类 号：R651.2[医药卫生—外科学]