大黄对小鼠肠粘膜屏障保护作用的机理探讨  

Study on the Mechanism by Which Rhubarb Protects the Mucosal Barrier of Intestine of Mouse

作　　者：李玉[1] 陈晓理[2] 张正[3] 黄兴兰[3] 闫兆平[3] 

机构地区：[1]四川大学华西医院肿瘤中心生物治疗科 [2]四川大学华西医院普外科 [3]四川大学华西医院消化科

出　　处：《四川大学学报（医学版）》2005年第2期210-212,共3页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金 (批准号 3 9970 93 2 )资助

摘　　要：目的　研究大黄对小鼠小肠 型磷脂酶 A2 ( 型 PL A2 )、溶菌酶分泌的影响。方法　 4 0只健康 ICR小鼠随机分为实验组及对照组。分别给予大黄汤剂、生理盐水灌胃 ,每 8h一次 ,每次 0 .3ml。 2 4 h后处死小鼠 ,切取小肠。用 10 0 g/ L 乙酸溶液冲洗肠腔、收集灌洗液及制备小肠组织匀浆。分别测定灌洗液和组织匀浆中 型PL A2 活力、溶菌酶含量。结果　小肠灌洗液中的 型 PL A2 活力和溶菌酶含量实验组比对对照组明显高 (P<0 .0 1) ;小肠匀浆中的 型 PL A2 活力、溶菌酶含量实验组比对照组明显低 (P<0 .0 1)。结论　大黄能刺激小鼠小肠肠壁分泌 型 PL A2 、溶菌酶 ,使肠腔内的 型 PL A2 、溶菌酶含量增加 ,增强肠粘膜屏障。Objective To observe the influence of Rhubarb on the the excretion of Type Ⅱ PLA 2 and lysozyme of small intestine of mouse. Methods Forty ICR mice were randomized to two groups. In the experimen group, the mice were gavaged with 0.3 ml 10% Rhubarb decoction every 8 hours; in the control group, the mice were given normal saline instead of Rhubarb decoction. After 24 hours, the mice were subjected to cervical dislocation, and their jejunum and ileum were taken out. The lumen of each resected intestine was rinsed with 100 g/L acetic acid, and the washed intestines from each mouse were cut into pieces 1-2 mm in length. Then the perfusate and homogenate were prepared, lyophilized, sealed, and stored at -20 ℃. The TypeⅡPLA 2 activity and lysozyme were assayed respectively. Results The Type ⅡPLA 2 activities and lysozyme of homogenate in rhubarb group were lower than those in Saline group(P<0.01). The typeⅡPLA 2 activities and lysozyme of perfusate in Rhubarb group were higher than those in Saline group(P<0.01). Conclusion Rhubarb can stimulate the small intestine of mice to excrete Type ⅡPLA 2 and lysozyme, thus resulting in the increase of Type ⅡPLA 2 and lysozyme contents in the intestinal tract and enhancing the function of mucosal barrier of intestine.

关 键 词：Ⅱ型PLA 溶菌酶 大黄 小鼠 肠粘膜屏障 

分 类 号：R285[医药卫生—中药学]