百合总皂甙的提取工艺  

Extraction Process of Total Saponins from Lilium Brownii

作　　者：任凤莲[1] 邱昌桂[1] 连琰[1] 

机构地区：[1]中南大学化学化工学院,湖南长沙410083

出　　处：《中南大学学报（自然科学版）》2005年第1期69-72,共4页Journal of Central South University:Science and Technology

摘　　要：以百合中总皂甙含量为考察指标,应用正交设计对百合总皂甙提取工艺中的温度、乙醇体积分数、固液比、回流时间和提取次数进行了研究;以薯蓣皂甙为标准,香草醛高氯酸为显色剂,冰醋酸为溶剂,用紫外可见分光光度计在波长为540 nm处对百合总皂甙的含量进行了测定。得出百合总皂甙的最佳提取工艺条件是:温度为 70 ℃,乙醇体积分数为80%,固液比为1∶6,提取时间为3 h,提取次数为3次;百合中总皂甙的含量为3.48 mg/g。The orthogonal design was used to optimize the extraction process of total saponins from lilium brownii, five factors in extraction were optimized, i.e., temperature, volume fraction of ethanol, the ratio of solid to liquid of extraction system, reflux time and extraction times. The dioscin was used as a standard compound, the vanillin-perchloric acid as chromogenic agent, glacial acetic acid as solvent, the content of total saponins determined with UV-756 spectrophotometer at 540 nm. The result shows that the optimum extraction condition obtained is as follows: temperature of 70°C, alcohol volume fraction of 80%, the ratio of solid to liquid of 1:6, reflux time of 3 h and extraction times of 3 times. And the content of total saponin in lilium brownii is 3.48 mg/g.

关 键 词：百合 总皂甙 提取 工艺 分光光度法 

分 类 号：R284.2[医药卫生—中药学]