不同应变对成骨细胞增殖活性及功能状态的影响  

Effects of different strain on the proliferation and function of osteoblasts

作　　者：张西正[1] 李彬[1] 郭勇[1] 吴金辉[1] 

机构地区：[1]军事医学科学院卫生装备研究所,天津300161

出　　处：《生物医学工程与临床》2005年第2期69-72,F002,共5页Biomedical Engineering and Clinical Medicine

基　　金：国家自然科学基金资助项目(10172093);全军医药卫生"十五"科研基金资助项目(01MA088)

摘　　要：目的了解力学因素在骨组织重建过程中的作用,探讨应变对成骨细胞增殖活性及功能状态的影响,为组织工程中研究应变对骨组织重建的影响提供理论依据。方法通过对大鼠成骨细胞施加不同的应变,采用免疫组织化学方法,观察应变作用下体外培养的大鼠成骨细胞增殖活性及功能状态。结果与结论在1000με、2000με应变的作用下,细胞增殖及碱性磷酸酶和FOS蛋白表达发生改变。Objective To study the role of mechanical factor in bone tissue remodeling,and the affecting activities and differentiation of osteoblast after loading mechanical strain,in order to provide the theoretical basis in tissue engineering for study the mechanism of strain in bone remodeling. Methods The different strains were loaded on the osteoblasts of rat,and the proliferation activities and function of osteoblasts was observed with immunohistochemical technique in vitro. Results and conclusion Under the mechanical strain of 1 000 με,2 000 με, the cellular proliferation,alkaline phosphatase and FOS albumen expression of osteoblast were changed in experiments.

关 键 词：成骨细胞 功能状态 增殖活性 骨组织 大鼠 影响 免疫组织化学方法 结论 理论依据 过程 

分 类 号：R318.0[医药卫生—生物医学工程]