乙醇对绵羊卵母细胞孤雌激活的研究  

Parthenogenetic Activation of Sheep Oocytes by Ethanol

作　　者：张云生[1] 张永胜[2] 皮文辉[3] 万鹏程[1] 石国庆[3] 杨永林[3] 刘守仁[3] 

机构地区：[1]石河子大学动物科技学院,新疆石河子832000 [2]新疆石河子紫泥泉绵羊研究所,新疆石河子832000 [3]新疆农垦科学院畜牧兽医研究所,新疆石河子832000

出　　处：《草食家畜》2005年第1期36-39,共4页Grass-Feeding Livestock

基　　金：国家高新技术研究发展计划"863"项目项目编号:2001AA243021

摘　　要：试验采用乙醇法孤雌激活绵羊体外成熟卵母细胞,研究了卵母细胞体外成熟时间、6-DMAP处理时间、乙醇浓度以及激活时间,对绵羊卵母细胞孤雌激活效果的影响。结果表明:(1)卵母细胞成熟22h、24h和26h卵裂率分别为60.7%、75.2%和74.5%,卵母细胞成熟24h的卵裂率较22h卵裂率,具有明显差异(P<0.05),与26h卵裂率差异不显著(P>0.05);其桑椹胚率38.6%,明显高于26h的桑椹胚率27.3%(P<0.05)。(2)6-DMAP6h的处理组,卵裂率83.4%,较2h卵裂率70.3%,有明显差异(P<0.05),也高于4h卵裂率79.4%,但差异不显著(P>0.05)。(3)乙醇浓度7%,激活3min、5min、7min卵裂率分别为76.7%、81.4%和70.4%,差异均不显著(P>0.05);激活3min囊胚率13.3%,显著高于激活7min囊胚率9.3%(P<0.05)。(4)乙醇浓度7%,激活绵羊卵母细胞5min,卵裂率85.5%,与乙醇浓度为5%的卵裂率72.2%,有显著差异(P<0.05),桑椹胚率和囊胚率,差异分别不显著(P>0.05)。In this study, it was designed to carry out parthenogenetic activation of sheep oocytes derived from IVM under various time of mature culture, 6-DMAP co-culture , activation time, also with various concentration of ethanol. For the first experiment, the results show that when oocytes cultivated for maturation with 22h,24h and 26h, the cleavage rate using ethanol activation were 60.7%,75.2% and 74.5% respectively and that cleavage rate matured with 24h was significantly higher than that of matured with 22h, but there was no significant difference between group of 24h and 26h(75.2%vs74.5%, P>0.05). The morula rate of oocytes matured with 24h was significantly higher than that of oocytes matured with 26h(38.6%vs27.3%, P<0.05). For the second experiment, the results show that the cleavage rate of oocytes co-cultured with 2mmol/L 6-DMAP for 6h was significant higer than that of co-cultured with 2mmol/L 6-DMAP for 2h　(83.4%vs70.3%, P<0.05) and there was no significant difference between group of 6h and 4h　(83.4%vs79.4%, P<0.05).For the third experiment, the cleavage rate was no significantly different between activation time of 3min group,5min group and 7min group(76.7%,81.4%,70.4%, respectly, P>0.05). The blastocyst rate of 3min group was significantly higher than that of 7min group(13.3%vs9.3%, P<0.05).For the forth experiment, the cleavage rate of oocytes activated with 7% ethanol comcentration was significantly higher than that of 5% ethanol concentration(85.5%vs72.2%, P<0.05), but both of morula rate and blastocyst rate among three group were no significantly difference.

关 键 词：卵裂率 绵羊 囊胚率 桑椹胚 孤雌激活 卵母细胞体外成熟 6-DMAP 乙醇浓度 处理 效果 

分 类 号：S823[农业科学—畜牧学] S826[农业科学—畜牧兽医]