RAPD Analysis for Genetic Variation within the Endangered Quillwort Isoetes hypsophila (Isoetaceae)  

作　　者：CHENJin-ming WANGJing-yuan LIUXing GituruWahitiRobert WANGQing-feng 

机构地区：[1]LaboratoryofPlantSystematicsandEvolutionaryBiology／CollegeofLifeSciences,WuhanUniversity,Wuhan430072,Hubei,China [2]BotanyDepartment,KenyattaUniversity,P.O.Box43844,Nairobi,Kenya

出　　处：《Wuhan University Journal of Natural Sciences》2005年第2期455-459,共5页武汉大学学报（自然科学英文版）

基　　金：Supported by the National Natural Science Foundation of China (30370098) and the State Key Basic Research and De velopment Plan (G2000046805)

摘　　要：The genetic variation in the critically endangered speciesIsoetes hypsophila was investigated using Random Amplified Polymorphism DNA (RAPD) markers. Thirteen primers were screened from sixty primers, and a total of 104 DNA fragments were scored, of which, 52 were polymorphic loci. Low-level genetic diversity within populations with PPB values ranging from 7.69%to 25.96%was found. An Anal ysis of Molecular Variance (AMOVA) indicated that the most of variance (78.30%) occurred between Yunnan and Si chuan. The variances among populations within regions and within populations were only 3.89%and 17.82%, respectively.The genetic variation in the critically endangered speciesIsoetes hypsophila was investigated using Random Amplified Polymorphism DNA (RAPD) markers. Thirteen primers were screened from sixty primers, and a total of 104 DNA fragments were scored, of which, 52 were polymorphic loci. Low-level genetic diversity within populations with PPB values ranging from 7.69%to 25.96%was found. An Anal ysis of Molecular Variance (AMOVA) indicated that the most of variance (78.30%) occurred between Yunnan and Si chuan. The variances among populations within regions and within populations were only 3.89%and 17.82%, respectively.

关 键 词：Key words conservation ENDANGERED genetic diversity Isoetes hypsophila RAPI 

分 类 号：Q949.363[生物学—植物学] Q347