健骨冲剂对去势大鼠骨组织基因差异表达影响的研究  

Study on the Differentially Expressed Genes in Bone Tissue of Ovariectomized Rats after Intervention with Chinese Herbs

作　　者：马宇滢[1] 张新民[1] 李世峰[1] 顾一峰[1] 陈伟华[1] 

机构地区：[1]复旦大学中西医结合研究所华山医院,上海200040

出　　处：《中国中西医结合杂志》2005年第2期138-142,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No30070541)

摘　　要：目的 探索健骨冲剂对绝经后骨质疏松症疗效的作用机制。方法 采用差异显示反转录-PCR(DDRT-PCR)方法,寻找骨组织内与健骨冲剂疗效相关的有差异表达的基因。结果 共筛选出10条有差异表达的基因片段,经克隆、测序,BLAST搜索,有9条差异基因片断在Genebank中找到高同源性的对应序列,其中2条是已知功能基因的mRNA,即透明质酸调节的运动受体(hyaluronan mediated motility receptor,RHAMM)和Na+、K+ATP转运酶β3亚单位(ATP1b3)。用半定量RT-PCR证实,这两个差异片段在健骨冲剂作用后表达分别下降和上调。结论 中药健骨冲剂对骨质疏松症疗效的作用机制可能与这两个蛋白质有关。To explore the therapeutic effect and mechanism of Jiangu granule (JGG) on postmenopausal osteoporosis. Methods Differential display reverse transcription PCR (DDRT-PCR) was used to detect the differentially expressed genes in bone tissues associated with therapeutic effect of JGG. Results Ten differentially expressed gene fragments were found, 9 of them, after cloning, sequencing and BLAST searching, were proved to be matched with highly homologous sequences in Genebank. Among them, two were known proteins: hyaluronan-mediated motility receptor (RHAMM) and ATPase, Na , K transporting beta 3 polypeptide (ATP1b3) . It was confirmed by semi-quantitative RT-PCR that these two gene fragments could be down- and up- regulated by JGG respectively. Conclusion The therapeutic effect of JGG in treating postmenopausal osteoporosis might be related with its regulation on the two kinds of protein, RHAMM and ATP1b3.

关 键 词：冲剂 骨组织 去势大鼠 疗效 作用机制 差异表达 绝经后骨质疏松症 结论 已知 方法 

分 类 号：R285[医药卫生—中药学]