松口蘑与假松口蘑ITS序列测定和分析比较  

SEQUENCE ANALYSIS OF THE INTERNAL TRANSCRIBED SPACER OF GENE CODING FOR rRNA IN TRICHOLOMA MATSUTAKE AND TRICHOLOMA BAKAMATSUTAKE

作　　者：沙涛[1] 丁骅孙[2] 李觅[2] 张汉波[1,3] 程立忠[2] 赵之伟[1] 张亚平[1,4] 

机构地区：[1]云南大学生物资源保护与利用国家重点实验室培育基地 [2]云南大学生物学系 [3]云南大学生物学系昆明650091 [4]中国科学院昆明动物研究所昆明650223

出　　处：《菌物学报》2005年第1期48-52,共5页Mycosystema

基　　金："十五"国家科技攻关计划"生物资源与生物安全技术研究开发"项目(批准号:No.2001BA707B01);中央级科研院所科技基础性工作专项--重要野生食用真菌种质资源收集与保藏研究

摘　　要：对松口蘑和假松口蘑进行ITS序列测序,通过DNAStar软件比较分析,发现松口蘑与假松口蘑的5.8SrDNA序列完全一致,ITS1和ITS2呈现不同程度的多态性。松口蘑ITS序列长度为601bp,假松口蘑ITS序列长度为563bp。设计了扩增松口蘑和假松口蘑ITS1的特异性引物,能够快速地区别松口蘑与假松口蘑。The Internal Transcribed Spacer of Gene Coding for rRNA was sequenced and analyzed. No difference was observed between 5.8S rDNA sequences of Tricholoma matsutake and Tricholoma bakamatsutake, but the length discrepancy and sequence polymorphism of ITS1 and ITS2 were existed between the two species. The length of entire ITS of Tricholoma matsutake was 601bp, and Tricholoma bakamatsutake, 563bp. Specific primers were designed for PCR ITS1 of the two species. It was easy to distinguish the two species by use of the specific primers.

关 键 词：松口蘑 ITS序列 特异性引物 长度 ITS2 测序 多态性 ITS1 DNA序列 发现 

分 类 号：S646[农业科学—蔬菜学]