Diethyldithiocarbamate inhibits iNOS expression in human lens epithelial cells stimulated by IFN-gamma and LPS  

作　　者：De-xinLI Si-lingWANG YoshimasaITO Jing-haiZHANG Chun-fuWU 

机构地区：[1]DepartmentofPharmaceutics,CollegeofPharmacy,ShenyangPharmaceuticalUniversity,Shenyang110016,China [2]SchoolofPharmacy//PharmaceuticalResearchandTechnologyInstitute,KinkiUniversity,Osaka577-8502,Japan

出　　处：《Acta Pharmacologica Sinica》2005年第3期359-363,共5页中国药理学报（英文版）

摘　　要：Aim:To investigate the biological activity of human lens epithelial cells (HLEC) in producing inducible nitric oxide synthase (iNOS) and nitric oxide (NO),and to assesse the effect of diethyldithiocarbamate (DDC) on iNOS mRNA levels and expression of NOS.Methods:The human lens epithelial cell line SRA 01/04 was used in this experiment.Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to detect,respectively,iNOS mRNA expression and protein production.Results:A costimulation by inter- feron gamma (IFN-γ) and lipopolysaccharide (LPS) was necessary for iNOS ex- pression in HLEC.The expression of iNOS was significantly reduced in a dose- dependent manner by adding DDC from 10 μmol/L to 1 mmol/L.Conclusion: The expression of iNOS in HLEC needs co-stimulation with IFN-γ and LPS and it is inhibited by DDC.Aim:To investigate the biological activity of human lens epithelial cells (HLEC) in producing inducible nitric oxide synthase (iNOS) and nitric oxide (NO),and to assesse the effect of diethyldithiocarbamate (DDC) on iNOS mRNA levels and expression of NOS.Methods:The human lens epithelial cell line SRA 01/04 was used in this experiment.Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to detect,respectively,iNOS mRNA expression and protein production.Results:A costimulation by inter- feron gamma (IFN-γ) and lipopolysaccharide (LPS) was necessary for iNOS ex- pression in HLEC.The expression of iNOS was significantly reduced in a dose- dependent manner by adding DDC from 10 μmol/L to 1 mmol/L.Conclusion: The expression of iNOS in HLEC needs co-stimulation with IFN-γ and LPS and it is inhibited by DDC.

关 键 词：human lens epithelial cells inducible nitric oxide synthase interferon gamma LIPOPOLYSACCHARIDE DIETHYLDITHIOCARBAMATE 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]