马鞍山、苏州地区婴幼儿轮状病毒腹泻临床特征和G血清型研究  

Study on Clinical Features of Rotavirus Diarrhea and G Type Serum in Infants and Children in Maanshan and Suzhou Areas

作　　者：宋晓波[1] 陶方标[2] 丁慧[1] 王蓓[3] 顾红英[4] 

机构地区：[1]马鞍山钢铁集团公司医院儿科 [2]安徽医科大学合肥230032 [3]东南大学公共卫生学院 [4]苏州儿童医院

出　　处：《实用儿科临床杂志》2005年第3期208-210,共3页Journal of Applied Clinical Pediatrics

摘　　要：目的　研究马鞍山和苏州地区婴幼儿轮状病毒 (RV)腹泻的临床特征和G血清型。方法　收集马鞍山和苏州地区5岁以下儿童急性腹泻粪便标本 1 2 67份 ,采用酶联免疫吸附试验和巢式 聚合酶链反应检测RV感染和血清型。结果　 1 2 67份标本中RV阳性 378份 ,血清型G型是优势株共 2 50株 ,其中G1 1 0 0株 ,G3 1 1 1株 ,G2 8株 ,G4、G9各 3株 ,混合感染 4株 ,未能分型2 1株 (8.40 % )。马鞍山地区以G1型为主 (58.54 % ) ,苏州地区以G3型为优势株 (47.85 % )。结论　RV是导致马鞍山和苏州地区婴幼儿腹泻的主要病原 ,流行的血清型分别为G1和G3型。Objective To investigate the clinical features and distribution of G types of rotavirus diarrhea in infants and children in Suzhou and Maanshan areas.Methods 1267 stool specimens were collected from children under 5 years with acute diarrhea. Enzyme-linked immunosorbant assay (ELISA) and nested polymeras chain reaction (Nested-PCR) were used to detect rotavirus and serotypes.Results In 1267 stool specimens, 378 were detected RV positive, and serotype G was the prevalent type in the two cities with number of 250 and the G3 and G1 types were the most prevalent which accounted for 40%(100/250) and 44.40 % (111/250), respectively. While the G2 ,G4, G9 types and mixed infections were 8,3,3,4 and the un-identified type was 8.40%(21/250). G1 type was the most prevalent in Maanshan are which accounted for 58.54% in all cases,while the G3 type accounted for the most of case(47.85%) in Suzhou area.Conclusions The results indicated that rotavirus was the most important etiologic agent in Maanshan and Suzhou areas,G1 and G3 were the most prevalent types,respectively. J Appl Clin Pediatr,2005,20(3):208-210

关 键 词：腹泻 儿童 轮状病毒 血清型 G型 

分 类 号：R725.1[医药卫生—儿科]