中药治疗对β-珠蛋白基因簇位点调控区高敏位点2与核蛋白结合作用的影响  

Effect of Chinese Herbs on β-Globin Gene Cluster Locus Control Hepersensitive site 2 Site Binding with Nucleoprotein

作　　者：柴立民[1] 吴志奎[1] 张新华[2] 刘志昂[2] 蔡辉国[3] 

机构地区：[1]中国中医研究院广安门医院分子生物学研究室,北京100053 [2]中国人民解放军第303医院血液病研究室,南宁200021 [3]中国医学科学院实验血液学国家重点实验室,天津530021

出　　处：《实用儿科临床杂志》2005年第3期239-241,i002,共4页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金资助 (30 1 71 1 99);国家自然科学基金重大研究计划资助 (90 4 0 90 0 3)

摘　　要：目的　探讨中药益髓生血颗粒调控 β- 珠蛋白mRNA的分子机制。 方法　中药治疗 3个月 ,分别提取正常人和患者治疗前后骨髓有核细胞的核蛋白 ;采用凝胶滞后实验 ,将核蛋白与HS2位点序列探针结合后 ,经聚丙烯酰胺凝胶电泳分离 ,观察电泳图谱的改变。结果　患者治疗前的核蛋白与探针结合后的电泳速率与正常人存在明显差异 ,而患者治疗后核蛋白与探针结合后与正常人的电泳图谱接近。结论　中药治疗后影响 β- 珠蛋白基因簇位点调控区HS2位点与核蛋白因子GATA 1的结合作用 ,这可能是中药治疗本病的分子机制之一。Objective To explore molecular mechanism and the curative effect of Yisuishengxue powder and its function of the hepersensitive site 2 (HS2) in β-globin gene cluster locus control region binding with nucleoprotein.Methods After 3 months treatment of Yisuishengxue powder, nucleoprotein was extracted from the morrow cell before and after treatment. The HS2 DNA probes was combined with nucleoproteins.Electrophoresis gel mobile lag was utilized for observing the mobile velocity of DNA segment.Observe the mobile velocity of DNA probes.Results The mobile velocity of probes combined with nucleoproteins before treatment was different form that of the controls, while it was very close to the controls after treatment.Conclusions It is suggested that this compounding medicine might affect the DNA segment of HS2 site in β-LCR binding with nucleoprotein GATA-1, which may be one molecular mechanism of Chinese herb therapy.

关 键 词：中医药 β珠蛋白基因簇位点调控区 DNASEI 高敏位点2 核蛋白 结合作用 

分 类 号：R272[医药卫生—中医儿科学]